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MB Sample ID: SA237394
| Local Sample ID: | WT2 |
| Subject ID: | SU002466 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6N |
| Age Or Age Range: | 6-weeks old |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002466 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6N |
| Age Or Age Range: | 6-weeks old |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| WT2 | SA237394 | FL029405 | Wild-type | Genotype |
Collection:
| Collection ID: | CO002459 |
| Collection Summary: | Mice were deeply anesthetized and perfused intracardially with saline containing 1 mM MOPS (pH 7.4). Liver was rapidly removed, frozen on liquid nitrogen and stored at –80°C until further processing. |
| Sample Type: | Liver |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002478 |
| Treatment Summary: | No treatment in this study. |
Sample Preparation:
| Sampleprep ID: | SP002472 |
| Sampleprep Summary: | Tissues with internal standards were homogenized with ice-cold methanol and extracted by the Bligh–Dyer method using chloroform and methanol to separate and recover the water-soluble fractions. The supernatant was filtered through a 5-kDa cut-off filter (Ultrafree-MC-PLHCC, Human Metabolome Technologies) at 9,100 × g for 2 h at 4 °C, dried in a centrifugal evaporator at 1,500 rpm at 1,000 Pa, and resuspended in 50 μl of ultrapure water before metabolome analysis. |
Combined analysis:
| Analysis ID | AN003873 | AN003874 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent 7100 CE | Agilent 7100 CE |
| Column | Fused silica capillary i.d. 50 μm × 80 cm | Fused silica capillary i.d. 50 μm × 80 cm |
| MS Type | ESI | ESI |
| MS instrument type | Other | Other |
| MS instrument name | Agilent 6224 TOF | Agilent 6490 QQQ |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | nmol | nmol |
Chromatography:
| Chromatography ID: | CH002870 |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | Fused silica capillary i.d. 50 μm × 80 cm |
| Chromatography Type: | CE |
MS:
| MS ID: | MS003614 |
| Analysis ID: | AN003873 |
| Instrument Name: | Agilent 6224 TOF |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | The spectrometer was scanned from m/z 50 to 1,000. Peaks were extracted using automatic integration software MasterHands (ver.2.16.0.15 Keio University). Putative metabolites were assigned based on the m/z value and migration time using Human Metabolomic Technologies’ standard library and known–unknown peak library. Peak areas were normalized to internal standards and sample amounts. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003615 |
| Analysis ID: | AN003874 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | The spectrometer was scanned from m/z 50 to 1,000. Peaks were extracted using automatic integration software (MassHunter Quantitative Analysis B.06.00, Agilent Technologies). Putative metabolites were assigned based on the m/z value and migration time using Human Metabolomic Technologies’ standard library and known–unknown peak library. Peak areas were normalized to internal standards and sample amounts. |
| Ion Mode: | NEGATIVE |
