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MB Sample ID: SA238955
| Local Sample ID: | D_d14 |
| Subject ID: | SU002483 |
| Subject Type: | Bacteria |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002483 |
| Subject Type: | Bacteria |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| D_d14 | SA238955 | FL029767 | 14 | Day |
| D_d14 | SA238955 | FL029767 | 1%DSS | Treatment |
Collection:
| Collection ID: | CO002476 |
| Collection Summary: | Samples were taken at day 1 and as a multiple of 24 h during the control phase (day 12-14) and the early (day 15) and late treatment phase (day 18 to day 20) from the bioreactors under unaerobic conditions. |
| Sample Type: | Feces |
Treatment:
| Treatment ID: | TR002495 |
| Treatment Summary: | Faecal pellets were isolated from the colon under aseptic conditions. Directly after collection, faecal pellets were stored at anaerobic conditions. The murine microbiota was cultivated in the chemostats from day 1 to day 13. At day 14, the bioreactors B, D and F were spiked with 1% DSS. |
Sample Preparation:
| Sampleprep ID: | SP002489 |
| Sampleprep Summary: | In brief, 20 µl of each sample was mixed with ACN to a final concentration of 50 %. SCFAs were derivatized with 200 mM 3-nitrophenylhydrazine and 120 mM N-(3-dimethylaminopropyl)-N‘-ethylcarbodiimide hydrochloride in pyridine and then diluted in 10 % ACN. |
Combined analysis:
| Analysis ID | AN003900 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | NEGATIVE |
| Units | µM |
Chromatography:
| Chromatography ID: | CH002887 |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003639 |
| Analysis ID: | AN003900 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | For identification and quantitation, a scheduled MRM method was used, with specific transitions for every SCFA. Data acquisition and peak integration were performed in Analyst® software (Version 1.6.2). Calculation of concentration was done using external calibration curves and statistics were performed using the R software programme. |
| Ion Mode: | NEGATIVE |
