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MB Sample ID: SA240420
| Local Sample ID: | P_732 |
| Subject ID: | SU002493 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 18-99 |
| Gender: | Male and female |
| Human Inclusion Criteria: | Hospitalized COVID-19 patients |
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Subject:
| Subject ID: | SU002493 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 18-99 |
| Gender: | Male and female |
| Human Inclusion Criteria: | Hospitalized COVID-19 patients |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| P_732 | SA240420 | FL030277 | M | Sex |
| P_732 | SA240420 | FL030277 | o | Variant |
| P_732 | SA240420 | FL030277 | COVID-19_positive | Group |
| P_732 | SA240420 | FL030277 | mild | Severity |
Collection:
| Collection ID: | CO002486 |
| Collection Summary: | The analysed were done on EDTA-plasma. All the samples were collected, processed and stored following the ISO standards (ISO 23118:2021), designed for high quality biological samples for metabolomic analysis. |
| Sample Type: | Blood (plasma) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002505 |
| Treatment Summary: | Not Applicable |
Sample Preparation:
| Sampleprep ID: | SP002499 |
| Sampleprep Summary: | Frozen EDTA-plasma samples were thawed at room temperature and shaken before use. A total of 350 μL of sodium phosphate buffer (70 mM Na2HPO4; 20% (v/v) 2H2O; 6.1 mM NaN3, 4.6 mM sodium trimethylsilyl [2,2,3,3−2H4] propionate (TMSP), pH 7.4) was added to 350 μL of each serum sample; the mixture was homogenized by vortexing for 30 s. A total of 600 μL of each mixture was transferred into a 5.00 mm NMR tube (Bruker BioSpin) for the analysis. |
Analysis:
| MB Sample ID: | SA240420 |
| Analysis ID: | AN003918 |
| Analysis Type: | NMR |
| Num Factors: | 16 |
| Num Metabolites: | 139 |
| Units: | Relative abundance |
NMR:
| NMR ID: | NM000258 |
| Analysis ID: | AN003918 |
| Instrument Name: | Bruker 600 MHz |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | 5-mm PATXI 1H-13C-15N and 2H-decoupling probe |
| NMR Solvent: | H2O |
| NMR Tube Size: | 5 mm |
| Pulse Sequence: | noesygp1D |
| Water Suppression: | water peak suppression-presaturation |
| Receiver Gain: | 90.5 |
| Temperature: | 310 K |
| Number Of Scans: | 32 |
| Dummy Scans: | 4 |
| Acquisition Time: | 2.7s |
| Relaxation Delay: | 4s |
| Spectral Width: | 18,028 Hz |
| Num Data Points Acquired: | 98000 |
| Line Broadening: | 0.3 |
| Baseline Correction Method: | automatic |
| Chemical Shift Ref Std: | doublet at 5.24 ppm |
| Binned Increment: | 0.02 ppm |
| Binned Data Excluded Range: | 4.5-5.5 ppm |
