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MB Sample ID: SA246278
| Local Sample ID: | 20210712_Palaemon_Branchie_M8_78_01_10986 |
| Subject ID: | SU002550 |
| Subject Type: | Other organism |
| Subject Species: | Palaemon serratus |
| Taxonomy ID: | 645159 |
| Age Or Age Range: | Adults |
| Weight Or Weight Range: | 10g |
| Height Or Height Range: | 5cm |
| Gender: | Male and female |
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Subject:
| Subject ID: | SU002550 |
| Subject Type: | Other organism |
| Subject Species: | Palaemon serratus |
| Taxonomy ID: | 645159 |
| Age Or Age Range: | Adults |
| Weight Or Weight Range: | 10g |
| Height Or Height Range: | 5cm |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 20210712_Palaemon_Branchie_M8_78_01_10986 | SA246278 | FL030888 | Gills | Organ |
| 20210712_Palaemon_Branchie_M8_78_01_10986 | SA246278 | FL030888 | Male | sex |
Collection:
| Collection ID: | CO002543 |
| Collection Summary: | Specimens of Palaemon serratus were collected by a professional fisherman (DYFLO) in February 2021, using special traps in the 2-nautical mile zone of the Cap of La Hève (Le Havre, Normandy, France). The collected prawns were returned to the laboratory within a maximum of 1 h, using 30 L-polypropylene tanks filled with artificial sea water (ASW; salinity of 33‰ from dissolution of TETRA SEA® salt, which is commonly used in maintenance of marine aquarium), in oxygenated conditions (Rollin et al., 2021). The prawns were transferred and kept into 80 L-aquariums that were connected to an ASW supply, and under constant aeration and filtration. Then, 10 mature males and gravid females (i.e. ovarian stage 4) of homogenous size (i.e. 8.5 ± 0.9 cm total length) and homogenous moult stage (i.e. D1’-D1’’) were anesthetized on ice and dissected. The gills, the nervous gland (i.e. eyestalks medulla and supra oesophageal ganglia), the muscle, the hepatopancreas and the gonads were weighted, individually frozen in liquid nitrogen and stored at -20°C. |
| Sample Type: | Shrimp organs |
| Collection Method: | Fishing net |
| Collection Location: | Le Havre, France |
| Storage Conditions: | -20℃ |
| Storage Vials: | eppendorfs |
Treatment:
| Treatment ID: | TR002562 |
| Treatment Summary: | no treatment was applied. Individuals were anaesthetized and organs were collected, frozen and kept at -20°C prior to metabolite extraction. |
Sample Preparation:
| Sampleprep ID: | SP002556 |
| Sampleprep Summary: | Metabolites were extracted from wet tissues with 75%-25% UHPLC methanol-water (with a ratio of 1 ml for 100 mg of fresh tissues), grinded mechanically (GLH850 OMNI) and sonicated with an ultrasonic probe (SONICS VibraCell, 130 Watt, 20 Khz, 60% amplitude) for 30 sec, twice. Samples were then centrifuged at 15,000 g for 10 min at 4°C and the supernatant collected and store at -20°C until mass spectrometry analysis. |
| Processing Storage Conditions: | -20℃ |
| Extract Storage: | -20℃ |
Combined analysis:
| Analysis ID | AN004014 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Bruker ELUTE UHPLC |
| Column | Thermo Acclaim Polar Advantage II (150 x 3mm,3um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Bruker Compact |
| Ion Mode | POSITIVE |
| Units | counts |
Chromatography:
| Chromatography ID: | CH002965 |
| Chromatography Summary: | The metabolites were analysed by injection of 2 µL of the supernatants on an UHPLC (ELUTE, Bruker) using a Polar Advances II 2.5 pore C18 column (Thermo, Waltham, MA, USA) at a 300 µL·min−1 flow rate with a linear gradient of acetonitrile in 0.1% formic acid (5%–90% in 16 min). |
| Instrument Name: | Bruker ELUTE UHPLC |
| Column Name: | Thermo Acclaim Polar Advantage II (150 x 3mm,3um) |
| Column Temperature: | 40 |
| Flow Gradient: | 5-90% |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% water |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003761 |
| Analysis ID: | AN004014 |
| Instrument Name: | Bruker Compact |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Metabolites were subsequently ionised and analysed using an electrospray ionization hybrid quadrupole time-of-flight high-resolution mass spectrometer (ESI-Qq-TOF Compact, Bruker) at 2 Hz speed on the 50–1500 m/z range on positive autoMS/MS mode between 2 and 8 Hz speed on the 50–1500 m/z range with information-dependent acquisition (IDA). |
| Ion Mode: | POSITIVE |
