Return to study ST002463 main page
MB Sample ID: SA246436
Local Sample ID: | Batch3_std1 |
Subject ID: | SU002553 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002553 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Batch3_std1 | SA246436 | FL030901 | 1 | diluted factor |
Collection:
Collection ID: | CO002546 |
Collection Summary: | Serum samples from conventional and GF mice were collected and stored at -80°C. |
Sample Type: | Blood (serum) |
Treatment:
Treatment ID: | TR002565 |
Treatment Summary: | Thawed on ice, 25 µL of serum sample, was mixed with 100 µL extraction solvent of ice-cold methanol containing indole-3-acetic acid-d4 and kynurenic acid-d5. Mixture was vortexed and incubated at −20 °C for 30 min. Following centrifugation at 12,000 × g for 15 min at 4℃, 90 µL supernatant was collected and subsequently evaporated to dryness (Thermo Scientific, Waltham, MA) and dissolved in 45 µL of 10% acetonitrile containing 1 µM chlorpropamide. After centrifugation at 12,000 × g for 15 min at 4℃, supernatants were transferred to autosampler vials for LC-MS analysis. |
Sample Preparation:
Sampleprep ID: | SP002559 |
Sampleprep Summary: | GF mice were reared in standard, sterile (autoclaved cages containing sterile (autoclaved) bedding inside a hermetically sealed isolator with HEPA-filtered airflow and maintained on sterile (autoclaved) water and sterile (autoclaved) food (LabDiet® 5013, Land O’Lakes) ad libitum. One of two groups of conventional C57BL/6J mice with ad libitum access to a standard laboratory chow were switched onto a semi-purified diet (AIN-93G) while the second remained on standard chow. After one week, portal vein blood was collected. |
Combined analysis:
Analysis ID | AN004018 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Shimadzu 20AD |
Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple TOF |
MS instrument name | ABI Sciex 5600 TripleTOF |
Ion Mode | POSITIVE |
Units | uM |
Chromatography:
Chromatography ID: | CH002968 |
Instrument Name: | Shimadzu 20AD |
Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
Column Temperature: | 55 |
Flow Gradient: | The initial condition were 97% A and 3 % B, increasing to 45% B at 10 min, 75% B at 12 min where it was held at 75% B until 17.5 min before returning to the initial conditions. |
Flow Rate: | 0.25 mL/min |
Solvent A: | Water with 0.1% formic acid |
Solvent B: | Acetonitrile with 0.1% formic acid |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS003765 |
Analysis ID: | AN004018 |
Instrument Name: | ABI Sciex 5600 TripleTOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
MS Comments: | Peakview was used for data analysis. |
Ion Mode: | POSITIVE |