Return to study ST002475 main page
MB Sample ID: SA247887
Local Sample ID: | PP10 |
Subject ID: | SU002565 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002565 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
PP10 | SA247887 | FL031168 | Placebo | treatment |
Collection:
Collection ID: | CO002558 |
Collection Summary: | The first pooped fecal sample in a day were collected during 3 days after the indicated time points and stored at -80 oC immediately for further research |
Sample Type: | Feces |
Treatment:
Treatment ID: | TR002577 |
Treatment Summary: | Out of those 60 students with anxiety and depression facing UNGEE, 30 were selected randomly as the PB group to receive the probiotic product; another 30 received placebo product were regarded as the P group. The 30 un-anxious students were assigned as the healthy control group (HP group). |
Sample Preparation:
Sampleprep ID: | SP002571 |
Sampleprep Summary: | Stool weighing 100 mg was added with 400 ml extract of methanol-acetonitrile-water (2:2:1, vol/vol/vol), vortexed and centrifuged at 14,000 g for 20min, and the supernatant was collected for further measurement. |
Combined analysis:
Analysis ID | AN004042 | AN004043 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | Triple TOF | Triple quadrupole |
MS instrument name | ABI Sciex 6600 TripleTOF | ABI Sciex 6600 TripleTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | Original peak intensity values | Original peak intensity values |
Chromatography:
Chromatography ID: | CH002990 |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um) |
Column Temperature: | 25 |
Flow Gradient: | The gradient elution program was as follows: 0 --- 0.5 min, 95% B; 0.5 --- 7 min, B varied linearly from 95% to 65 %; 7 --- 8 min, B varied linearly from 65% to 40%; 8---9 min, B was maintained at 40%; 9---9.1 min, B varied linearly from 40% to 95%; 9.1--12 min, B maintained at 95%. |
Flow Rate: | 0.5 mL/min |
Solvent A: | 100% water; 25 mM ammonium acetate; 25 mM ammonia |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |
MS:
MS ID: | MS003789 |
Analysis ID: | AN004042 |
Instrument Name: | ABI Sciex 6600 TripleTOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
MS Comments: | Ion Source Gas1(Gas1):60,Ion Source Gas2(Gas2):60,Curtain gas (CUR):30,source temperature:600℃,IonSapary Voltage Floating (ISVF) The raw data in Wiff format was converted to .mzXML format by ProteoWizard and then converted to .mzXML format using XCMS software. |
Ion Mode: | POSITIVE |
MS ID: | MS003790 |
Analysis ID: | AN004043 |
Instrument Name: | ABI Sciex 6600 TripleTOF |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Ion Source Gas1(Gas1):60,Ion Source Gas2(Gas2):60,Curtain gas (CUR):30,source temperature:600℃,IonSapary Voltage Floating (ISVF) The raw data in Wiff format was converted to .mzXML format by ProteoWizard and then converted to .mzXML format using XCMS software. |
Ion Mode: | NEGATIVE |