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MB Sample ID: SA254743
| Local Sample ID: | T-A6 |
| Subject ID: | SU002632 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002632 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| T-A6 | SA254743 | FL032641 | vehicle+cisplatin | Genotype |
| T-A6 | SA254743 | FL032641 | WT | Strain |
Collection:
| Collection ID: | CO002625 |
| Collection Summary: | 8 weeks old C57BL/6 male mice were randomly divided into three groups: control group (control; n=8), cisplatin-induced AKI group (cisplatin; n=8), and cisplatin plus TRAF1 plasmid injection group (cisplatin + TRAF1; n=8). Both cisplatin and cisplatin + TRAF1 groups were given 20 mg/kg cisplatin intraperitoneal (i.p.) injection, while the control group received the same amount of saline i.p. injection. The mice in cisplatin + TRAF1 group were pretreated with TRAF1 performed by a hydrodynamic-based gene delivery approach 36 h before cisplatin administration. Mice in the three groups were sacrificed after cisplatin treatment for 72 h. The kidney tissues were cut horizontally and collected. |
| Collection Protocol Filename: | protocol.pdf |
| Sample Type: | Kidney |
Treatment:
| Treatment ID: | TR002644 |
| Treatment Summary: | 8 weeks old C57BL/6 male mice were randomly divided into three groups: control group (control; n=8), cisplatin-induced AKI group (cisplatin; n=8), and cisplatin plus TRAF1 plasmid injection group (cisplatin + TRAF1; n=8). Both cisplatin and cisplatin + TRAF1 groups were given 20 mg/kg cisplatin intraperitoneal (i.p.) injection, while the control group received the same amount of saline i.p. injection. The mice in cisplatin + TRAF1 group were pretreated with TRAF1 performed by a hydrodynamic-based gene delivery approach 36 h before cisplatin administration. Mice in the three groups were sacrificed after cisplatin treatment for 72 h. The kidney tissues were cut horizontally and collected. |
| Treatment Protocol Filename: | protocol.pdf |
Sample Preparation:
| Sampleprep ID: | SP002638 |
| Sampleprep Summary: | 1. Take all samples into a 2 mL EP tube, add 2 steel balls, put them into the tissue grinder, grind at 50 Hz for 60 s, and then homogenize the samples; 2. Accurately weigh 100 mg (±1%) of the homogenized sample into a 2 mL EP tube, add accurately 0.6mL of methanol (including internal standard), and vortex for 30 s 3. Add 2 steel balls, put them into the tissue grinder, and grind for 60 s at 50 Hz; 4. Centrifuge at 4 ℃ for 10 min at 12000 rpm, and the supernatant was filtered through 0.22 µm membrane to obtain the prepared samples for LC-MS; 5. Take 20 µL from each sample to the quality control (QC) samples; (These QC samples were used to monitor deviations of the analytical results from these pool mixtures and compare them to the errors caused by the analytical instrument itself) 6. Use the rest of the samples for LC-MS detection. |
| Sampleprep Protocol Filename: | protocol.pdf |
Combined analysis:
| Analysis ID | AN004166 | AN004167 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Ultimate 3000 | Thermo Ultimate 3000 |
| Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Focus | Thermo Q Exactive Focus |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH003085 |
| Methods Filename: | protocol.pdf |
| Instrument Name: | Thermo Ultimate 3000 |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0~1 min, 2% B/D; 1~9 min, 2%~50% B/D; 9~12 min, 50%~98% B/D; 12~13.5 min, 98% B/D; 13.5~14 min, 98%~2% B/D; 14~20 min, 2% D-positive model (14~17 min, 2% B-negative model) |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 0.1% formic acid in water and 0.1% formic acid in acetonitrile |
| Solvent B: | 5 mM ammonium formate in water and acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003913 |
| Analysis ID: | AN004166 |
| Instrument Name: | Thermo Q Exactive Focus |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | protocol.pdf |
| MS ID: | MS003914 |
| Analysis ID: | AN004167 |
| Instrument Name: | Thermo Q Exactive Focus |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | protocol.pdf |
