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MB Sample ID: SA256114
| Local Sample ID: | D_Serum_10 |
| Subject ID: | SU002650 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002650 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| D_Serum_10 | SA256114 | FL032759 | Control | Factor |
Collection:
| Collection ID: | CO002643 |
| Collection Summary: | This study was approved by the Asan Institutional Review Board (IRB No. 2019-1017). The inclusion criteria for the cohort were as follows: adult patients aged > 18 years, patients admitted to the medical intensive care unit (ICU) and clinically diagnosed as sepsis according to the Sepsis-2 definition16, and those enrolled within 48 hours of ICU admission. The patients' serum was collected at the time of sepsis cohort enrollment. The healthy control registry included healthy people who visited the Health Screening and Promotion Center at Asan Medical Center. The diagnosis of direct ARDS and indirect ARDS was made through a blind review by the two intensivists reaching the same judgment.Since the eligible ARDS patients belonged to the sepsis cohort, the direct ARDS group included pulmonary ARDS patients with pneumonia-induced sepsis, and the indirect ARDS included extrapulmonary ARDS patients with non-pneumonia induced sepsis. |
| Sample Type: | Blood (serum) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002662 |
| Treatment Summary: | not available |
Sample Preparation:
| Sampleprep ID: | SP002656 |
| Sampleprep Summary: | 50 μL human serum was mixed well, and 50 μL of internal standard solutions (50 nM C17 ceramide for sphingolipid; 200 nM 18:0 D70 PC and 1 μM 16:0 D31-18:1 PE for plasmalogen and PC/PE profiling) were added. Metabolites were extracted from aqueous and organic phases by liquid-liquid extraction after the addition of chloroform and H2O. Nonpolar metabolites containing lipids were collected from the lower organic phase. The organic phases were dried under vacuum and stored at -20℃oC until further analysis. The dried matter was reconstituted with 50 μL of MeOH prior to LC-MS/MS analysis. |
Combined analysis:
| Analysis ID | AN004198 | AN004199 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 | Agilent 1290 |
| Column | Agilent ZORBAX Eclipse Plus C18 (50 x 2.1mm,1.8um) | Agilent Pursuit 5 C18 (150 x 2.1mm,5um) |
| MS Type | ESI | ESI |
| MS instrument type | QTRAP | QTRAP |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE | POSITIVE |
| Units | nM | nM |
Chromatography:
| Chromatography ID: | CH003111 |
| Chromatography Summary: | LC-MS/MS (MRM) for phospholipid |
| Instrument Name: | Agilent 1290 |
| Column Name: | Agilent ZORBAX Eclipse Plus C18 (50 x 2.1mm,1.8um) |
| Column Temperature: | 30 |
| Flow Gradient: | isocratic condition of 60% B |
| Flow Rate: | 400 µL/min |
| Solvent A: | 10 mM ammonium acetate in MeOH/isopropanol/H2O(900/50/50) |
| Solvent B: | 10 mM ammonium acetate in MeOH/isopropanol/H2O(940/50/10) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003112 |
| Chromatography Summary: | LC-MS/MS (MRM) for sphingolipid |
| Instrument Name: | Agilent 1290 |
| Column Name: | Agilent Pursuit 5 C18 (150 x 2.1mm,5um) |
| Column Temperature: | 30 |
| Flow Gradient: | 50% A for 0 min, 50% A for 5 min, 50 to 30% A for 3 min, 30% A for 7 min, 30 to 10% A for 7 min, 10% A for 3 min, 10 to 50% A for 0.1 min, and 50% A for 4.9 min |
| Flow Rate: | 200 µL/min |
| Solvent A: | 5 mM ammonium formate/MeOH/tetrahydrofuran(500/200/300) |
| Solvent B: | 5 mM ammonium formate/MeOH/tetrahydrofuran(100/200/700) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003945 |
| Analysis ID: | AN004198 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Data analysis was performed using Analyst 1.5.2 software. Extracted ion chromatograms (EIC) corresponding to each metabolite were used for quantitation (MRM). The area under the curve of each EIC was normalized to that of the internal standard. For metabolites related to PC/PE profiling, the peak area ratio of each metabolite to that of the internal standard was used for a relative comparison. For plasmalogens, the calibration range was generally 0.1 nM to 10 μM with R2>0.99 |
| Ion Mode: | POSITIVE |
| MS ID: | MS003946 |
| Analysis ID: | AN004199 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Data analysis was performed using Analyst 1.5.2 software. Extracted ion chromatograms (EIC) corresponding to each metabolite were used for quantitation (MRM). The area under the curve of each EIC was normalized to that of the internal standard. For ceramides and sphingomyelins, the calibration range was generally 0.1 nM to 10 μM with R2>0.99 |
| Ion Mode: | POSITIVE |
