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MB Sample ID: SA259166
| Local Sample ID: | L1-3 |
| Subject ID: | SU002686 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002686 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| L1-3 | SA259166 | FL033499 | ALDH1L1 | Transgene |
Collection:
| Collection ID: | CO002679 |
| Collection Summary: | ALDH1L1-expressing cells or control (empty vector) cells were cultured in DMEM medium containing 5% fetal bovine serum and metabolites were collected during growing phase. |
| Sample Type: | HuH-7 cells |
Treatment:
| Treatment ID: | TR002698 |
| Treatment Summary: | No treatment in this study. |
Sample Preparation:
| Sampleprep ID: | SP002692 |
| Sampleprep Summary: | Cells were washed with 5%(w/w) mannitol solution, and then extracted with methanol. Extracts were filtered through a 5-kDa cut-off filter (Ultrafree-MC-PLHCC, Human Metabolome Technologies) at 9,100 × g for 2 h at 4 °C, dried in a centrifugal evaporator at 1,500 rpm at 1,000 Pa, and resuspended in 50 μl of ultrapure water before metabolome analysis. |
Combined analysis:
| Analysis ID | AN004255 | AN004256 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent CE | Agilent CE |
| Column | Agilent Fused silica capillary (80cm x 50um) | Agilent Fused silica capillary (80cm x 50um) |
| MS Type | ESI | ESI |
| MS instrument type | Other | Other |
| MS instrument name | Agilent CE-TOFMS | Agilent CE-TOFMS |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Relative Area | relative area |
Chromatography:
| Chromatography ID: | CH003161 |
| Chromatography Summary: | CE-TOFMS (ESI positive) |
| Instrument Name: | Agilent CE |
| Column Name: | Agilent Fused silica capillary (80cm x 50um) |
| Column Temperature: | 20 |
| Flow Gradient: | - |
| Flow Rate: | +30 kV |
| Solvent A: | 1 M formic acid |
| Solvent B: | - |
| Chromatography Type: | CE |
| Chromatography ID: | CH003162 |
| Chromatography Summary: | CE-TOFMS (ESI negative) |
| Instrument Name: | Agilent CE |
| Column Name: | Agilent Fused silica capillary (80cm x 50um) |
| Column Temperature: | 20 |
| Flow Gradient: | - |
| Flow Rate: | −30 kV |
| Solvent A: | 50 mM ammonium acetate |
| Solvent B: | - |
| Chromatography Type: | CE |
MS:
| MS ID: | MS004002 |
| Analysis ID: | AN004255 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | The spectrometer was scanned from m/z 50 to 1,000. Peaks were extracted using automatic integration software MasterHands (ver.2.16.0.15 Keio University). Signal peaks corresponding to isotopomers, adduct ions, and other product ions of known metabolites were excluded, and remaining peaks were annotated with putative metabolites from the HMT metabolite database based on their migration time and m/z values. Peak areas were normalized to internal standards and sample amounts. |
| Ion Mode: | POSITIVE |
| MS ID: | MS004003 |
| Analysis ID: | AN004256 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | The spectrometer was scanned from m/z 50 to 1,000. Peaks were extracted using automatic integration software MasterHands (ver.2.16.0.15 Keio University). Signal peaks corresponding to isotopomers, adduct ions, and other product ions of known metabolites were excluded, and remaining peaks were annotated with putative metabolites from the HMT metabolite database based on their migration time and m/z values. Peak areas were normalized to internal standards and sample amounts. |
| Ion Mode: | NEGATIVE |
