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MB Sample ID: SA259182
Local Sample ID: | A11_011 |
Subject ID: | SU002687 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002687 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
A11_011 | SA259182 | FL033502 | Turmor bearing-Zn treated | treatment |
Collection:
Collection ID: | CO002680 |
Collection Summary: | At sacrifice, esophagi from Zn-treated vs Zn-untreated rats were isolated (n = 10 rats per cohort). Esophageal epithelium was prepared by using a blade to remove the submucosal and muscularis layers, snap-frozen in liquid nitrogen, and stored at –80 °C. Frozen esophageal samples from Zn-untreated and Zn-treated rats (20 mg per esophagus) were shipped on dry ice to the NIH West Coast Metabolomics Center, University of California, Davis. |
Sample Type: | Epithelial cells |
Treatment:
Treatment ID: | TR002699 |
Treatment Summary: | Two treatments, 10 rats per treatment group Untreated group was given drinking water Zn-treated group was given Zn-gluconate in the drinking water. |
Sample Preparation:
Sampleprep ID: | SP002693 |
Sampleprep Summary: | SOP_blood-GCMS-06052020.pdf |
Combined analysis:
Analysis ID | AN004257 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Leco Pegasus IV GC |
Column | Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um) |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Leco Pegasus HT TOF |
Ion Mode | POSITIVE |
Units | normalized peak heights |
Chromatography:
Chromatography ID: | CH003163 |
Instrument Name: | Leco Pegasus IV GC |
Column Name: | Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um) |
Column Temperature: | 50 - 300 |
Flow Gradient: | 1 ml/min |
Flow Rate: | 1 ml/min |
Solvent A: | Helium |
Solvent B: | Helium |
Chromatography Type: | GC |
MS:
MS ID: | MS004004 |
Analysis ID: | AN004257 |
Instrument Name: | Leco Pegasus HT TOF |
Instrument Type: | GC-TOF |
MS Type: | EI |
MS Comments: | Acquisition of masses between 85-500 Scan rate 17 spec/sec Processing with in house software |
Ion Mode: | POSITIVE |