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MB Sample ID: SA260074
| Local Sample ID: | 90265015810 |
| Subject ID: | SU002703 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002703 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 90265015810 | SA260074 | FL033688 | Control | Group |
| 90265015810 | SA260074 | FL033688 | 8 weeks of training or control time | Timepoint |
| 90265015810 | SA260074 | FL033688 | Female | Sex |
Collection:
| Collection ID: | CO002696 |
| Collection Summary: | - |
| Sample Type: | Heart |
Treatment:
| Treatment ID: | TR002715 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP002709 |
| Sampleprep Summary: | Nucleotides are extracted from 200”l of tissue homogenates prepared at 50 mg /ml in methanol using hexane. The extracts are dried under nitrogen and reconstituted in water. |
| Sampleprep Protocol Filename: | pass1b_experimental_design_metabolomics.pdf |
Combined analysis:
| Analysis ID | AN004273 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity UPLC system |
| Column | Chromolith FastGradient RP-18e 50-2mm column (EMD Millipore, Billerica, MA, USA) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Waters Xevo TQ-XS |
| Ion Mode | NEGATIVE |
| Units | pmol/mg of tissue |
Chromatography:
| Chromatography ID: | CH003179 |
| Chromatography Summary: | Injection volume is 2 ”L. Mobile phase A is 95% water, 5% methanol and 5 mM dimethylhexylamine adjusted to pH 7.5 with acetic acid. Mobile phase B is 20% water, 80% methanol and 10 mM dimethylhexylamine. Flow rate is set to 0.3 mL/min and column temperature is 40 °C. A 22 min gradient method (t=0, %B=0; t=1.2, %B=0; t=22, %B=40) is run followed by a 3 min wash and 7 min equilibration. |
| Methods Filename: | pass1b_nuc_methods.pdf |
| Instrument Name: | Waters Acquity UPLC system |
| Column Name: | Chromolith FastGradient RP-18e 50-2mm column (EMD Millipore, Billerica, MA, USA) |
| Column Temperature: | 40C |
| Flow Gradient: | 22-minute gradient (t=0, %B=0; t=1.2, %B=0; t=22, %B=40) followed by a 3-minute wash and 7-minute equilibration |
| Flow Rate: | 0.3 ml/min |
| Solvent A: | 95% water/5% methanol; 5 mM dimethylhexylamine adjusted to pH 7.5 with acetic acid |
| Solvent B: | 20% water/80% methanol; 10 mM dimethylhexylamine |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004020 |
| Analysis ID: | AN004273 |
| Instrument Name: | Waters Xevo TQ-XS |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | All nucleotides were detected in negative ion MRM mode based on a characteristic fragmentation reaction. |
| Ion Mode: | NEGATIVE |
