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MB Sample ID: SA261315

Local Sample ID:90441016910
Subject ID:SU002723
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

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Subject:

Subject ID:SU002723
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
90441016910SA261315FL033938TrainingGroup
90441016910SA261315FL0339382 weeks of trainingTimepoint
90441016910SA261315FL033938MaleSex

Collection:

Collection ID:CO002716
Collection Summary:-
Sample Type:Brown adipose

Treatment:

Treatment ID:TR002732
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002729
Sampleprep Summary:homogenized in PBS, isotope IS added, deproteinized, 2 steps derivatization with ethoxamine, MTBSTFA
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004293
Analysis type MS
Chromatography type GC
Chromatography system Agilent Intuvo 9000 GC
Column Agilent DB5-MS (30m x 0.25mm, 0.25um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5977B HES MSD
Ion Mode POSITIVE
Units nano moles per vial

Chromatography:

Chromatography ID:CH003199
Chromatography Summary:Analytes were chromatographically separated with 17 minutes GC gradient
Methods Filename:pass1b_tca_methods.pdf
Instrument Name:Agilent Intuvo 9000 GC
Column Name:Agilent DB5-MS (30m x 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS004040
Analysis ID:AN004293
Instrument Name:Agilent 5977B HES MSD
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:SIM; peak integration, concentrations calculated
Ion Mode:POSITIVE
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