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MB Sample ID: SA261413
| Local Sample ID: | 90564017010 |
| Subject ID: | SU002725 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002725 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 90564017010 | SA261413 | FL033959 | Training | Group |
| 90564017010 | SA261413 | FL033959 | 1 week of training or control time | Timepoint |
| 90564017010 | SA261413 | FL033959 | Female | Sex |
Collection:
| Collection ID: | CO002718 |
| Collection Summary: | - |
| Sample Type: | White adipose |
Treatment:
| Treatment ID: | TR002734 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP002731 |
| Sampleprep Summary: | purification of oxylipids with SPE column |
| Sampleprep Protocol Filename: | pass1b_experimental_design_metabolomics.pdf |
Combined analysis:
| Analysis ID | AN004295 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | ExionLC AC |
| Column | ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 Qtrap |
| Ion Mode | POSITIVE |
| Units | ng/uL |
Chromatography:
| Chromatography ID: | CH003201 |
| Chromatography Summary: | Lipids were separated by liquid chromatography with 20 minutes gradient |
| Methods Filename: | pass1b_oxylipneg_etamidpos_methods.pdf |
| Instrument Name: | ExionLC AC |
| Column Name: | ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um) |
| Column Temperature: | 50C |
| Flow Gradient: | t=0 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=0.5 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=1 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2.1 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=5 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=7 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12.1 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=18 min: 90%/10% (%A/%B) @ 0.5 ml/min |
| Flow Rate: | 0.5 ml/min |
| Solvent A: | 100% water; 10 mM ammonium acetate |
| Solvent B: | 100% acetonitrile; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004042 |
| Analysis ID: | AN004295 |
| Instrument Name: | ABI Sciex 5500 Qtrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Multiple reaction monitoring mode (MRM) |
| Ion Mode: | POSITIVE |
