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MB Sample ID: SA263520
| Local Sample ID: | IPE_Reference_Heart |
| Subject ID: | SU002754 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002754 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| IPE_Reference_Heart | SA263520 | FL034389 | QC-Reference | Group |
| IPE_Reference_Heart | SA263520 | FL034389 | 0 hour | Timepoint |
| IPE_Reference_Heart | SA263520 | FL034389 | - | Sex |
Collection:
| Collection ID: | CO002747 |
| Collection Summary: | - |
| Sample Type: | Heart |
Treatment:
| Treatment ID: | TR002763 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP002760 |
| Sampleprep Summary: | IPA extraction with three cycles of freeze/thaw(with sonication) |
| Sampleprep Protocol Filename: | pass1b_experimental_design_metabolomics.pdf |
Combined analysis:
| Analysis ID | AN004324 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Vanquish horizon LC |
| Column | Thermo Accucore C30 (100 x 2.1mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH003230 |
| Chromatography Summary: | global lipid separation |
| Methods Filename: | pass1b_lrpneg_lrppos_methods.pdf |
| Instrument Name: | Vanquish horizon LC |
| Column Name: | Thermo Accucore C30 (100 x 2.1mm,2.6um) |
| Column Temperature: | 50C |
| Flow Gradient: | t=0 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=1 min: 40%/60% (%A/%B) @ 0.4 ml/min | t=5 min: 30%/70% (%A/%B) @ 0.4 ml/min | t=8 min: 10%/90% (%A/%B) @ 0.4 ml/min | t=8.2 min: 0%/100% (%A/%B) @ 0.4 ml/min | t=10.5 min: 0%/100% (%A/%B) @ 0.4 ml/min | t=10.7 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=12 min: 80%/20% (%A/%B) @ 0.4 ml/min |
| Flow Rate: | 0.4 ml/min |
| Solvent A: | 40% water/60% acetonitrile; 10 mM ammonium formate; 0.1% formic acid |
| Solvent B: | 10% acetonitrile/90% isopropanol; 10 mM ammonium formate; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004071 |
| Analysis ID: | AN004324 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
