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MB Sample ID: SA264290
| Local Sample ID: | 80013827002_3 |
| Subject ID: | SU002765 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002765 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 80013827002_3 | SA264290 | FL034530 | QC-ExternalStandard | Group |
| 80013827002_3 | SA264290 | FL034530 | 0 hour | Timepoint |
| 80013827002_3 | SA264290 | FL034530 | - | Sex |
Collection:
| Collection ID: | CO002758 |
| Collection Summary: | - |
| Sample Type: | White adipose |
Treatment:
| Treatment ID: | TR002774 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP002771 |
| Sampleprep Summary: | 500 ul of tissue homogenate preprared at 50 mg/ml in 50% acetonitrile/0.3% formic acid are spiked with C17 Acyl CoA. The acyl CoAs are purified by solid phase extraction using 2-(2-pyridyl) ethyl functionalized Silica gel (Sigma-Aldrich 54127-U). |
| Sampleprep Protocol Filename: | pass1b_experimental_design_metabolomics.pdf |
Combined analysis:
| Analysis ID | AN004335 |
|---|---|
| Analysis type | MS |
| Chromatography type | Flow induction analysis |
| Chromatography system | Waters Acquity UPLC |
| Column | No column |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Waters Xevo TQ-S |
| Ion Mode | POSITIVE |
| Units | pmol/mg of tissue |
Chromatography:
| Chromatography ID: | CH003241 |
| Chromatography Summary: | 80% methanol/20% water containing 30 mM ammonium hydroxide is used as the mobile phase, the flow is 0.030 ml/min. |
| Methods Filename: | pass1b_acoa_methods.pdf |
| Instrument Name: | Waters Acquity UPLC |
| Column Name: | No column |
| Chromatography Type: | Flow induction analysis |
MS:
| MS ID: | MS004082 |
| Analysis ID: | AN004335 |
| Instrument Name: | Waters Xevo TQ-S |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Ion ratios of endogenous acyl CoAs to the C-17 CoA internal standard are computed from centroided spectra using a software package NeoLynx (Waters, Milford, MA). The ratios are converted to concentrations using calibrators prepared by spiking tissue homogenates with authentic CoAs (Sigma, St. Louis , MO) having saturated acyl chain lengths C0- C18. Corrections for the heavy isotope effects, mainly 13C, to the adjacent m+2 spectral peaks in a particular chain length cluster are made empirically by referring to the observed spectra for the analytical standards. The values are expressed in pmol/mg. Spectra are acquired in the multichannel acquisition mode monitoring the neutral loss of 507 amu (phosphoadenosine diphosphate) and scanning from m/z 750 to1060. |
| Ion Mode: | POSITIVE |
