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MB Sample ID: SA264307

Local Sample ID:90587017012
Subject ID:SU002765
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

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Subject:

Subject ID:SU002765
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
90587017012SA264307FL034533TrainingGroup
90587017012SA264307FL0345332 weeks of trainingTimepoint
90587017012SA264307FL034533FemaleSex

Collection:

Collection ID:CO002758
Collection Summary:-
Sample Type:White adipose

Treatment:

Treatment ID:TR002774
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002771
Sampleprep Summary:500 ul of tissue homogenate preprared at 50 mg/ml in 50% acetonitrile/0.3% formic acid are spiked with C17 Acyl CoA. The acyl CoAs are purified by solid phase extraction using 2-(2-pyridyl) ethyl functionalized Silica gel (Sigma-Aldrich 54127-U).
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004335
Analysis type MS
Chromatography type Flow induction analysis
Chromatography system Waters Acquity UPLC
Column No column
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Waters Xevo TQ-S
Ion Mode POSITIVE
Units pmol/mg of tissue

Chromatography:

Chromatography ID:CH003241
Chromatography Summary:80% methanol/20% water containing 30 mM ammonium hydroxide is used as the mobile phase, the flow is 0.030 ml/min.
Methods Filename:pass1b_acoa_methods.pdf
Instrument Name:Waters Acquity UPLC
Column Name:No column
Chromatography Type:Flow induction analysis

MS:

MS ID:MS004082
Analysis ID:AN004335
Instrument Name:Waters Xevo TQ-S
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Ion ratios of endogenous acyl CoAs to the C-17 CoA internal standard are computed from centroided spectra using a software package NeoLynx (Waters, Milford, MA). The ratios are converted to concentrations using calibrators prepared by spiking tissue homogenates with authentic CoAs (Sigma, St. Louis , MO) having saturated acyl chain lengths C0- C18. Corrections for the heavy isotope effects, mainly 13C, to the adjacent m+2 spectral peaks in a particular chain length cluster are made empirically by referring to the observed spectra for the analytical standards. The values are expressed in pmol/mg. Spectra are acquired in the multichannel acquisition mode monitoring the neutral loss of 507 amu (phosphoadenosine diphosphate) and scanning from m/z 750 to1060.
Ion Mode:POSITIVE
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