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MB Sample ID: SA264897

Local Sample ID:90439016606
Subject ID:SU002774
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

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Subject:

Subject ID:SU002774
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
90439016606SA264897FL034644TrainingGroup
90439016606SA264897FL0346442 weeks of trainingTimepoint
90439016606SA264897FL034644MaleSex

Collection:

Collection ID:CO002767
Collection Summary:-
Sample Type:Lung

Treatment:

Treatment ID:TR002783
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP002780
Sampleprep Summary:See SOP
Sampleprep Protocol Filename:pass1b_experimental_design_metabolomics.pdf

Combined analysis:

Analysis ID AN004344
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity II
Column Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6545 QTOF
Ion Mode NEGATIVE
Units peak area

Chromatography:

Chromatography ID:CH003250
Methods Filename:pass1b_rpneg_rppos_ionpneg_methods.pdf
Instrument Name:Agilent 1290 Infinity II
Column Name:Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)
Column Temperature:55C
Flow Gradient:Linear ramp from 0% to 100% B from 0-10 minutes, hold 100% B until 17 minutes, linear return to 0% B from 17 to 17.1 minutes, hold 0% B until 20 minutes
Flow Rate:0.45 ml/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.025% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004091
Analysis ID:AN004344
Instrument Name:Agilent 6545 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:See SOP protocol MiCAS_SOP004_Data post-processing and QC_v2_20190904.pdf
Ion Mode:NEGATIVE
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