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MB Sample ID: SA265618
| Local Sample ID: | Control_KHGU- |
| Subject ID: | SU002783 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002783 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Control_KHGU- | SA265618 | FL034760 | Control | Group |
Collection:
| Collection ID: | CO002776 |
| Collection Summary: | Blood was drawn into fluoride tubes for glucose measurement, heparin tubes for lipid measurement, and EDTA tubes for HbA1c and metabolomics analyses. Plasma was isolated and stored at -80C until analysis. |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR002792 |
| Treatment Summary: | NA |
Sample Preparation:
| Sampleprep ID: | SP002789 |
| Sampleprep Summary: | Prior to LC-MS analysis, 10ul of plasma was resuspended in 9 volumes of pre-chilled (-20°C) methanol:acetonitrile:water (5:3:2, v:v) and vortexed continuously for 30 min at 4°C. Insoluble material was removed by centrifugation at 18,000 g for 10 min at 4°C and supernatants were isolated for metabolomics analysis by UHPLC-MS. |
Combined analysis:
| Analysis ID | AN004353 | AN004354 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Phenomenex Kinetex C18 (100 x 2.1mm,1.7um) | Phenomenex Kinetex C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | AU | AU |
Chromatography:
| Chromatography ID: | CH003259 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Phenomenex Kinetex C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 45 |
| Flow Gradient: | 0-0.5min 5% B; 1.1min 95% B; 2.75min 95%B; 3min 5%B; 5min 5%B |
| Flow Rate: | 0.45 |
| Solvent A: | 0.1% formic acid in water |
| Solvent B: | 0.1% formic acid in acetonitrile |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003260 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Phenomenex Kinetex C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 45 |
| Flow Gradient: | 0-0.5min 5% B; 1.1min 95% B; 2.75min 95%B; 3min 5%B; 5min 5%B |
| Flow Rate: | 0.45 |
| Solvent A: | 5% acetonitrile 1mM ammonium acetate |
| Solvent B: | 95% acetonitrile 1mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004100 |
| Analysis ID: | AN004353 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The Q Exactive mass spectrometer (ThermoFisher) was operated independently in positive or negative ion mode, scanning in Full MS mode (2 microscans) from 60 to 900 m/z at 70,000 resolution, with 4 kV spray voltage, 45 sheath gas, 15 auxiliary gas. Calibration was performed prior to analysis using the PierceTM Positive and Negative Ion Calibration Solutions (ThermoFisher). Acquired data was then converted from raw to mzXML file format using Mass Matrix (Cleveland, OH). Samples were analyzed in randomized order with a technical mixture injected periodically through analysis to qualify instrument performance. Metabolite assignments, isotopologue distributions, and correction for expected natural abundances of deuterium, 13C, and 15N isotopes were performed using MAVEN (Princeton, NJ) |
| Ion Mode: | POSITIVE |
| MS ID: | MS004101 |
| Analysis ID: | AN004354 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The Q Exactive mass spectrometer (ThermoFisher) was operated independently in positive or negative ion mode, scanning in Full MS mode (2 microscans) from 60 to 900 m/z at 70,000 resolution, with 4 kV spray voltage, 45 sheath gas, 15 auxiliary gas. Calibration was performed prior to analysis using the PierceTM Positive and Negative Ion Calibration Solutions (ThermoFisher). Acquired data was then converted from raw to mzXML file format using Mass Matrix (Cleveland, OH). Samples were analyzed in randomized order with a technical mixture injected periodically through analysis to qualify instrument performance. Metabolite assignments, isotopologue distributions, and correction for expected natural abundances of deuterium, 13C, and 15N isotopes were performed using MAVEN (Princeton, NJ) |
| Ion Mode: | NEGATIVE |
