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MB Sample ID: SA270681

Local Sample ID:ABC-4100
Subject ID:SU002805
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Subject:

Subject ID:SU002805
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
ABC-4100SA270681FL035193MaleSex
ABC-4100SA270681FL035193CONTROLDiagnosis

Collection:

Collection ID:CO002798
Collection Summary:spun inside centrifuge and processed
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR002814
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP002811
Sampleprep Summary:SOP blood-LCGCextract-02252019
Sampleprep Protocol Filename:SOP_blood-LCGCextract-02252019.docx

Combined analysis:

Analysis ID AN004376 AN004377
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity
Column Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6546 QTOF Agilent 6546 QTOF
Ion Mode POSITIVE NEGATIVE
Units normalized peak heights normalized peak heights

Chromatography:

Chromatography ID:CH003282
Instrument Name:Agilent 1290 Infinity
Column Name:Waters ACQUITY UPLC BEH C18 (50 x 2.1mm,1.7um)
Column Temperature:65°C
Flow Gradient:0 min 15% (B), 0–0.75 min 30% (B), 0.75-0.98 min 48% (B), 0.98-4.00 min 82% (B), 4.00-4.13 min 99% (B), 4.13-4.50 min 99% (B), 4.50-4.58 min 15% (B), 4.58-5.50 min 15% (B)
Flow Rate:0.8 mL/min
Solvent A:60% acetonitrile/40% water; 10 mM ammonium formate; 0.1% formic acid
Solvent B:90% isopropanol/10% acetonitrile; 10 mM ammonium formate; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004125
Analysis ID:AN004376
Instrument Name:Agilent 6546 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:The quadrupole/time-of-flight (QTOF) mass spectrometers are operated with electrospray ionization (ESI) performing full scan in the mass range m/z 65–1700. Agilent 6546 is equipped with a dual JetStream Source. modes producing both unique and complementary spectra. Instrument parameters are as follows (positive mode) Gas Temp 320°C, Gas Flow 8 l/min, Nebulizer 35 psi, Sheath Gas 350°C, Sheath Gas Flow 11, Capillary Voltage 3500 V, Nozzle Voltage 1000V, Fragmentor 175V, Skimmer 65V. Data (both profile and centroid) are collected at a rate of 5 scans per second. In negative ion mode, Gas Temp 360°C, Gas Flow 9 l/min, Fragmentor 120V, with the other parameters identical to positive ion mode. A reference solution generating ions of 121.050 and 922.007 m/z in positive mode and 119.036 and 966.0007 m/z in negative mode, and these are used for continuous mass correction.
Ion Mode:POSITIVE
  
MS ID:MS004126
Analysis ID:AN004377
Instrument Name:Agilent 6546 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:The quadrupole/time-of-flight (QTOF) mass spectrometers are operated with electrospray ionization (ESI) performing full scan in the mass range m/z 65–1700. Agilent 6546 is equipped with a dual JetStream Source. modes producing both unique and complementary spectra. Instrument parameters are as follows (positive mode) Gas Temp 320°C, Gas Flow 8 l/min, Nebulizer 35 psi, Sheath Gas 350°C, Sheath Gas Flow 11, Capillary Voltage 3500 V, Nozzle Voltage 1000V, Fragmentor 175V, Skimmer 65V. Data (both profile and centroid) are collected at a rate of 5 scans per second. In negative ion mode, Gas Temp 360°C, Gas Flow 9 l/min, Fragmentor 120V, with the other parameters identical to positive ion mode. A reference solution generating ions of 121.050 and 922.007 m/z in positive mode and 119.036 and 966.0007 m/z in negative mode, and these are used for continuous mass correction.
Ion Mode:NEGATIVE
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