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MB Sample ID: SA270920
| Local Sample ID: | CRS9 |
| Subject ID: | SU002806 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002806 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| CRS9 | SA270920 | FL035198 | stress | Treatment |
Collection:
| Collection ID: | CO002799 |
| Collection Summary: | To precipitate proteins, we combined 100 μl of SERUM with 800 μl of methanol, incubated at -20 for 30 min, then centrifuged at 18000 rpm for 10 min. Subsequently, 850 μl of supernatant was transferred to a new tube and evaporated to dryness in a Thermo Savant SpeedVac. Then 200 μl of resuspension solvent methanol was added, compounds were dissolved by vortexing for 5 min and samples were clarified by centrifuged at 18000 rpm for 10 min. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR002815 |
| Treatment Summary: | Chronic restraint stress (RS) in mice were placed inside a plastic mouse holder for 4 h daily, from 10:00 am to 14:00 pm, and repeated for a total of 14 consecutive days. During non-stress periods, all mice were conventionally housed and allowed free access to food and water. |
Sample Preparation:
| Sampleprep ID: | SP002812 |
| Sampleprep Summary: | To precipitate proteins, we combined 200 μl SERUM with 800 μl of methanol, incubated at -20 for 30 min, then centrifuged at 18000 rpm for 10 min. Subsequently, 850 μl of supernatant was transferred to a new tube and evaporated to dryness in a Thermo Savant SpeedVac. Then 200 μl of resuspension solvent methanol was added, compounds were dissolved by vortexing for 5 min and samples were clarified by centrifuged at 18000 rpm for 10 min. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -20℃ |
Combined analysis:
| Analysis ID | AN004378 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 6546 Q-TOF |
| Column | Waters Atlantis 3μm particle size T3 column (2.1 × 10 cm) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6545 QTOF |
| Ion Mode | NEGATIVE |
| Units | Abundance |
Chromatography:
| Chromatography ID: | CH003283 |
| Instrument Name: | Agilent 6546 Q-TOF |
| Column Name: | Waters Atlantis 3μm particle size T3 column (2.1 × 10 cm) |
| Column Temperature: | 40 |
| Flow Gradient: | A linear gradient of 0–95% eluent B over 10min. Eluent B was held at 100% for 2.5 min, then brought to 5% over 0.5 min and the column was re-equilibrated at 0% eluent B for 4 min |
| Flow Rate: | 0.25ml/min |
| Solvent A: | 100% water; 0.05% formic acid; 0.1% ammonium formate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004127 |
| Analysis ID: | AN004378 |
| Instrument Name: | Agilent 6545 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Fast data-dependent acquisition (DDA) MS/MS experiments were performed. The Progenesis QI (Nonlinear Dynamics, Newcastle, UK) was used for peak picking and alignment to screen the metabolic biomarkers that displayed significant changes between the control and the fructose-treated group. Molecular identification of the assigned biomarkers was accomplished by matching the acquired precursors and fragment ions against several standard metabolome databases including the Human Metabolome Database (http://www.hmdb.ca/), MassBank (http://www.massbank.jp/index.html), and METLIN (http://metlin.scripps.edu/index.php). Partial metabolite identification was further confirmed by comparison with available standards. Metabolic pathway enrichment analysis of these identified metabolic biomarkers was carried out by MetaboAnalyst 3.0 |
| Ion Mode: | NEGATIVE |
