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MB Sample ID: SA288655
| Local Sample ID: | SA019 |
| Subject ID: | SU002842 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 5-14 |
| Gender: | Males |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002842 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 5-14 |
| Gender: | Males |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| SA019 | SA288655 | FL035602 | DMD | Diagnosis |
| SA019 | SA288655 | FL035602 | - | steroid_status |
Collection:
| Collection ID: | CO002835 |
| Collection Summary: | Subjects are consented and given a bag containing a urine collection cup, antiseptic wipes and directions of how to give a clean catch urine sample. They are then lead to a restroom where they collect their own urine sample using a clean catch method (direction provided). Urine samples are then placed on ice and brought to the UF CTSI Biorepository. There, samples are transferred into 500uL aliquots and stored at -80C. Samples will remain there until samples will be removed and ran on the NMR for this study. |
| Sample Type: | Urine |
Treatment:
| Treatment ID: | TR002851 |
| Treatment Summary: | No treatment, samples are from a Natural History Clinical Trial. Samples are either boys with DMD or control boys all within the ages 5-14. Unaffected, control boys are moderately active (less than 3 low to moderate exercise bouts per week) |
Sample Preparation:
| Sampleprep ID: | SP002848 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | SB_AA_Assay.pdf, SB_OA_Assay.pdf |
Combined analysis:
| Analysis ID | AN004436 | AN004437 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Unspecified | Reversed phase |
| Chromatography system | Agilent 1290 Infinity HPLC | Dionex UltiMate 3000 HPLC |
| Column | AccQ·Tag 2 x 100 mm, 1.7 µm column, Waters Corp | Acquity UPLC BEH C18 2.1 x 100 mm, 1.7 µm column, Waters Corp |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ | Thermo Quantiva QQQ |
| Ion Mode | POSITIVE | POSITIVE |
| Units | umol/mg Creatinine | umol/mg creatinine |
Chromatography:
| Chromatography ID: | CH003332 |
| Chromatography Summary: | Amino acid assay |
| Methods Filename: | SB_AA_Assay.pdf |
| Instrument Name: | Agilent 1290 Infinity HPLC |
| Column Name: | AccQ·Tag 2 x 100 mm, 1.7 µm column, Waters Corp |
| Column Temperature: | - |
| Flow Gradient: | - |
| Flow Rate: | - |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | Unspecified |
| Chromatography ID: | CH003333 |
| Chromatography Summary: | Organic Acids assay |
| Methods Filename: | SB_OA_Assay.pdf |
| Instrument Name: | Dionex UltiMate 3000 HPLC |
| Column Name: | Acquity UPLC BEH C18 2.1 x 100 mm, 1.7 µm column, Waters Corp |
| Column Temperature: | - |
| Flow Gradient: | - |
| Flow Rate: | - |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004183 |
| Analysis ID: | AN004436 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | AA assay ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS004184 |
| Analysis ID: | AN004437 |
| Instrument Name: | Thermo Quantiva QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | OA assay- HESI |
| Ion Mode: | POSITIVE |
