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MB Sample ID: SA289499
| Local Sample ID: | UHBO-00909 |
| Subject ID: | SU002861 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6JRcc |
| Age Or Age Range: | 10-12 weeks |
| Animal Housing: | specific pathogen-free conditions |
| Animal Light Cycle: | 12-h light/dark cycle |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002861 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6JRcc |
| Age Or Age Range: | 10-12 weeks |
| Animal Housing: | specific pathogen-free conditions |
| Animal Light Cycle: | 12-h light/dark cycle |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| UHBO-00909 | SA289499 | FL035716 | CD_CD | MOTHER_DIET |
| UHBO-00909 | SA289499 | FL035716 | CD | OFFSPRING_DIET |
Collection:
| Collection ID: | CO002854 |
| Collection Summary: | Blood was withdrawn from the mouse and proceeded for serum extraction. The sera were stored at -80°C until shipped to Metabolon. Further preparation was done there. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR002870 |
| Treatment Summary: | Samples were taken from the developed Maternal Obesity model. This model was generated as described in the Material and Methods section. No additional treatment was done. |
Sample Preparation:
| Sampleprep ID: | SP002867 |
| Sampleprep Summary: | Sera were taken and sent to Metabolon. Further sample preparation was done on Metabolon Site. In short, samples were prepared using the automated MicroLab STAR® system from Hamilton Company. Several recovery standards were added prior to the first step in the extraction process for QC purposes. To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis. |
Combined analysis:
| Analysis ID | AN004468 | AN004469 | AN004470 | AN004471 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | HILIC |
| Chromatography system | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm, 1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | POSITIVE | NEGATIVE | NEGATIVE |
| Units | Peak Area | Peak Area | Peak Area | Peak Area |
Chromatography:
| Chromatography ID: | CH003353 |
| Chromatography Summary: | Low pH polar |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | - |
| Flow Gradient: | - |
| Flow Rate: | - |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003354 |
| Chromatography Summary: | Low pH Lipophilic |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | - |
| Flow Gradient: | - |
| Flow Rate: | - |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003355 |
| Chromatography Summary: | High pH |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | - |
| Flow Gradient: | - |
| Flow Rate: | - |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003356 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm, 1.7um) |
| Column Temperature: | - |
| Flow Gradient: | - |
| Flow Rate: | - |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004215 |
| Analysis ID: | AN004468 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Proprietary analytical software for integration and peak picking |
| Ion Mode: | POSITIVE |
| MS ID: | MS004216 |
| Analysis ID: | AN004469 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Proprietary analytical software for integration and peak picking |
| Ion Mode: | POSITIVE |
| MS ID: | MS004217 |
| Analysis ID: | AN004470 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Proprietary analytical software for integration and peak picking |
| Ion Mode: | NEGATIVE |
| MS ID: | MS004218 |
| Analysis ID: | AN004471 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Proprietary analytical software for integration and peak picking |
| Ion Mode: | NEGATIVE |
