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MB Sample ID: SA294434
| Local Sample ID: | N3 |
| Subject ID: | SU002879 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Age Or Age Range: | 9 weeks |
| Weight Or Weight Range: | 380-430 |
| Gender: | Male |
| Animal Animal Supplier: | Janvier |
| Animal Housing: | Grouped |
| Animal Light Cycle: | 12:12 (normal phase) |
| Animal Feed: | Ad libitum normal diet |
| Animal Water: | Ad libitum |
| Species Group: | Sprague-Dawley |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002879 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Age Or Age Range: | 9 weeks |
| Weight Or Weight Range: | 380-430 |
| Gender: | Male |
| Animal Animal Supplier: | Janvier |
| Animal Housing: | Grouped |
| Animal Light Cycle: | 12:12 (normal phase) |
| Animal Feed: | Ad libitum normal diet |
| Animal Water: | Ad libitum |
| Species Group: | Sprague-Dawley |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| N3 | SA294434 | FL035927 | Dark phase sample | factor |
Collection:
| Collection ID: | CO002872 |
| Collection Summary: | CSF was collected during natural light or dark phase (2 AM/PM) via cisterna magna punctur, spun down 10 minutes then put on dry ice until storage at -80C. |
| Collection Protocol Filename: | methods.txt |
| Sample Type: | Cerebrospinal fluid |
| Storage Conditions: | -80℃ |
| Collection Vials: | Eppendorf tube |
| Storage Vials: | Microtube, 72-730-006, Sarstedt AG & Co |
| Collection Tube Temp: | 4 |
Treatment:
| Treatment ID: | TR002888 |
| Treatment Summary: | No treatment, natural light and dark phase, anesthesia during sampling 6 mg/ml xylazine + 60 mg/ml ketamine. Animals was housed at light-controlled facility a minimum of 1½ weeks prior to experimentation. |
Sample Preparation:
| Sampleprep ID: | SP002885 |
| Sampleprep Summary: | No sample prep was done after freezing. |
Combined analysis:
| Analysis ID | AN004512 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF MS |
| Ion Mode | UNSPECIFIED |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003389 |
| Chromatography Summary: | HILIC and Reverse-phase was performed by MS-Omics (Denmark). Data recieved includ raw spectrometry files and combined data from the negative and positive mode (supplied in data section) |
| Chromatography Comments: | For Reverse phase column was Water acquity HSS T3 (2.1x150 mm, 1.7 um) coupled with a high-resolution quadrupole-orbitrap mass spectrometer |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Column Temperature: | 30 |
| Flow Gradient: | - |
| Flow Rate: | 300 uL/minute |
| Solvent A: | 100% water; 10 mM ammonium formate; 0.1% formic acid (pH 3.1) |
| Solvent B: | 100% methanol; 10 mM ammonium formate; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004259 |
| Analysis ID: | AN004512 |
| Instrument Name: | Thermo Q Exactive HF MS |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | iterative data-dependent acquisition, run to Data was processed using Compound Discoverer 3.2 (ThermoFisher Scientific) and Skyline. |
| Ion Mode: | UNSPECIFIED |
