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MB Sample ID: SA295620
| Local Sample ID: | Ctrl3 |
| Subject ID: | SU002881 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002881 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Ctrl3 | SA295620 | FL035935 | Ctrl | Genotype |
Collection:
| Collection ID: | CO002874 |
| Collection Summary: | Mice were anaesthetized with volatile anesthetic isoflurane (3-4% for induction, 1-2% for maintenance, in O2≈ 2 L/min). DRG (left L4–L5) were surgically exposed and diessected. Fresh DRG samples were frozen in liquid nitrogen.5-6 DRGs were collected as one sample |
| Sample Type: | DRG |
Treatment:
| Treatment ID: | TR002890 |
| Treatment Summary: | No treatment. |
Sample Preparation:
| Sampleprep ID: | SP002887 |
| Sampleprep Summary: | Fresh tissue samples were frozen in liquid nitrogen. After thawing on ice, 10 mg tissue was homogenized in -80°C precooled labeled-solution (MeOH: H2O=4:1, v/v) for 30 s, 4 cycles, and placed at -80°C for 30 minutes. In ice water bath, ultrasonic wave was used for 10 min and centrifuged at 14000 rpm. The precipitated protein was dissolved in no labeled-liquid (MeOH: H2O=4:1), vortexed and centrifuged. After derivatization with N-methyl-N-(trimethylsilyl)tri-fluoroacetamide for 30 min, the supernatant was detected by GC-MS. Metabolites were identified by means of KEGG Metabolic pathways. |
Combined analysis:
| Analysis ID | AN004515 | AN004516 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Nexera UHPLC LC-30A | Nexera UHPLC LC-30A |
| Column | Waters BEH Amide (2.1 X 100mm, 1.7μm) | Waters BEH Amide (2.1 X 100mm, 1.7μm) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Nexera UHPLC LC-30A | Nexera UHPLC LC-30A |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | pmoles/l | pmoles/l |
Chromatography:
| Chromatography ID: | CH003392 |
| Instrument Name: | Nexera UHPLC LC-30A |
| Column Name: | Waters BEH Amide (2.1 X 100mm, 1.7μm) |
| Column Temperature: | 40℃ |
| Flow Gradient: | 0～1.5min,90%B.1.5~5min,90-45%B.5~10min,45%B.10~11min,45~90B.11~23min,90B |
| Flow Rate: | 0.3ml/min |
| Solvent A: | 10% acetonitrile/90% water; 0.2% acetic acid |
| Solvent B: | 90% acetonitrile/10% water; 0.2% acetic acic |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004262 |
| Analysis ID: | AN004515 |
| Instrument Name: | Nexera UHPLC LC-30A |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | POSITIVE |
| MS ID: | MS004263 |
| Analysis ID: | AN004516 |
| Instrument Name: | Nexera UHPLC LC-30A |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | NEGATIVE |
