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MB Sample ID: SA300784
Local Sample ID: | T542 |
Subject ID: | SU002908 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002908 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
T542 | SA300784 | FL036534 | TNT | Treatment |
Collection:
Collection ID: | CO002901 |
Collection Summary: | Excision while the heart still beating and flash freeze within 15 seconds of excision |
Sample Type: | Heart |
Treatment:
Treatment ID: | TR002917 |
Treatment Summary: | Saline IP and heparine SC injection in control & mutant group. After 10 minutes of saline injection we performed cervical dislocation, whole heart excision and flash freeze within 15 seconds of excision. |
Sample Preparation:
Sampleprep ID: | SP002914 |
Sampleprep Summary: | 2 mg of lyophilized tissue extracted with 1 mL 3:3:2 ACN/IPA/H2O 450 uL of extract dried and derivatized Derivatization is 10 uL of MeOX + Pyridine + 90 uL MSTFA |
Combined analysis:
Analysis ID | AN004557 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890A |
Column | Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um) |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Leco Pegasus IV TOF |
Ion Mode | POSITIVE |
Units | Peak Heights normalized by mTIC |
Chromatography:
Chromatography ID: | CH003424 |
Instrument Name: | Agilent 7890A |
Column Name: | Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um) |
Column Temperature: | 50-330°C |
Flow Gradient: | NA |
Flow Rate: | 1mL/min |
Solvent A: | NA |
Solvent B: | NA |
Chromatography Type: | GC |
MS:
MS ID: | MS004304 |
Analysis ID: | AN004557 |
Instrument Name: | Leco Pegasus IV TOF |
Instrument Type: | GC-TOF |
MS Type: | EI |
MS Comments: | Mass spectrometer settings: A Leco Pegasus IV time of flight mass spectrometer is controlled by the Leco ChromaTOF software vs. 2.32 (St. Joseph, MI). The transfer line temperature between gas chromatograph and mass spectrometer is set to 280°C. Electron impact ionization at 70V is employed with an ion source temperature of 250°C. Acquisition rate is 17 spectra/second, with a scan mass range of 85-500 Da. |
Ion Mode: | POSITIVE |