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MB Sample ID: SA302527
| Local Sample ID: | T545 |
| Subject ID: | SU002924 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002924 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| T545 | SA302527 | FL036635 | Control TNT | Treatment |
Collection:
| Collection ID: | CO002917 |
| Collection Summary: | Excision while the heart still beating and flash freeze within 15 seconds of excision |
| Sample Type: | Heart |
Treatment:
| Treatment ID: | TR002933 |
| Treatment Summary: | Saline IP and heparine SC injection in control & mutant group. After 10 minutes of saline injection we performed cervical dislocation, whole heart excision and flash freeze within 15 seconds of excision. |
Sample Preparation:
| Sampleprep ID: | SP002930 |
| Sampleprep Summary: | 2 mg of lyophilized tissue extracted with 1 mL 3:3:2 ACN/IPA/H2O 450 uL of extract dried and resuspended in 80:20 ACN/H2O. |
Combined analysis:
| Analysis ID | AN004582 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1290 |
| Column | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple TOF |
| MS instrument name | ABI Sciex 6600 TripleTOF |
| Ion Mode | POSITIVE |
| Units | peak heights |
Chromatography:
| Chromatography ID: | CH003443 |
| Chromatography Summary: | Column: Waters Acquity UPLC BEH Amide Column, 1.7 μm, 2.1 mm x 150 mm); Pre-column: Waters Acquity UPLC BEH Amide VanGuard pre-column (1.7 μm , 5 mm × 2.1 mm;) |
| Instrument Name: | Agilent 1290 |
| Column Name: | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 40C |
| Flow Gradient: | 0 min 100% (B), 0-2 min 100% (B), 2-7 min 70% (B), 7.7-9 min 40% (B), 9.5-10.25 min 30% (B), 10.25- 12.75 min 100% (B), 16.75 min 100% (B) |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | Ultrapure water with 10 mM ammonium formiate + 0.125% formic acid, pH 3 |
| Solvent B: | 95:5 v/v acetonitrile:ultrapure water w/ 10 mM ammonium formiate + 0.125% formic acid, pH 3 |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004328 |
| Analysis ID: | AN004582 |
| Instrument Name: | ABI Sciex 6600 TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| MS Comments: | The triple time-of-flight (TTOF) mass spectrometers are operated with electrospray ionization (ESI) performing full scan in the mass range m/z 50-1500 in positive mode. Instrument parameters are as follows: Gas Temp 500°C, Ion Source Gas 1 50, Ion Source Gas 2 50, Curtain Gas 34, Ion Spray Voltage 4000 V. Data was collected in centroid mode, cycle time 0.5 seconds for a total of 1679 cycles. Full MS-ddMS/MS was acquired. |
| Ion Mode: | POSITIVE |
