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MB Sample ID: SA302610
| Local Sample ID: | MtdBlank004 |
| Subject ID: | SU002926 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002926 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| MtdBlank004 | SA302610 | FL036645 | Method Blank | Treatment |
Collection:
| Collection ID: | CO002919 |
| Collection Summary: | Excision while the heart still beating and flash freeze within 15 seconds of excision |
| Sample Type: | Heart |
Treatment:
| Treatment ID: | TR002935 |
| Treatment Summary: | Saline IP and heparine SC injection in control & mutant group. After 10 minutes of saline injection we performed cervical dislocation, whole heart excision and flash freeze within 15 seconds of excision. |
Sample Preparation:
| Sampleprep ID: | SP002932 |
| Sampleprep Summary: | 2 mg of lyophilized tissue extracted with 225 uL MeOH + 188 uL H2O + 750 uL MTBE. 350 uL of the organic layer was dried and resuspended 90:10 MeOH + Toluene with internal standards. |
Combined analysis:
| Analysis ID | AN004584 | AN004585 | AN004586 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 | Agilent 1290 | Waters Acquity |
| Column | Waters ACQUITY UPLC CSH C18 (100 x 1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (100 x 1mm,1.7um) | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | Orbitrap |
| MS instrument name | Agilent 6530 QTOF | Agilent 6550 QTOF | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
| Units | peak heights | peak heights | raw area counts rescaled to set the median equal to 1 |
Chromatography:
| Chromatography ID: | CH003445 |
| Chromatography Summary: | - |
| Instrument Name: | Agilent 1290 |
| Column Name: | Waters ACQUITY UPLC CSH C18 (100 x 1mm,1.7um) |
| Column Temperature: | 65C |
| Flow Gradient: | 0 min 15% (B), 0–2 min 30% (B), 2–2.5 min 48% (B), 2.5–11 min 82% (B), 11–11.5 min 99% (B), 11.5– 12 min 99% (B), 12–12.1 min 15% (B), 12.1–15 min 15% (B) |
| Flow Rate: | 0.6 mL/min |
| Solvent A: | 60:40 v/v acetonitrile:water + 10 mM ammonium formate + 0.1% formic acid |
| Solvent B: | 90:10 v/v isopropanol:acetonitrile + 10 mM ammonium formate + 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003446 |
| Chromatography Summary: | Low pH polar (LC/MS Pos early) |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | - |
| Flow Gradient: | - |
| Flow Rate: | - |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004330 |
| Analysis ID: | AN004584 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The quadrupole/time-of-flight (QTOF) mass spectrometers are operated with electrospray ionization (ESI) performing full scan in the mass range m/z 65–1700 in positive (Agilent 6530, equipped with a JetStreamSource) and negative (Agilent 6550, equipped with a dual JetStream Source) modes producing both unique and complementary spectra. Instrument parameters are as follows (positive mode) Gas Temp 325°C, Gas Flow 8 l/min, Nebulizer 35 psig, Sheath Gas 350°C, Sheath Gas Flow 11, Capillary Voltage 3500 V, Nozzle Voltage 1000V, Fragmentor 120V, Skimmer 65V. Data (both profile and centroid) are collected at a rate of 2 scans per second. In negative ion mode, Gas Temp 200°C, Gas Flow 14 l/min, Fragmentor 175V, with the other parameters identical to positive ion mode. For the 6530 QTOF, a reference solution generating ions of 121.050 and 922.007 m/z in positive mode and 119.036 and 966.0007 m/z in negative mode, and these are used for continuous mass correction. For the 6550, the reference solution is introduced via a dual spray ESI, with the same ions and continuous mass correction |
| Ion Mode: | POSITIVE |
| MS ID: | MS004331 |
| Analysis ID: | AN004585 |
| Instrument Name: | Agilent 6550 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The quadrupole/time-of-flight (QTOF) mass spectrometers are operated with electrospray ionization (ESI) performing full scan in the mass range m/z 65–1700 in positive (Agilent 6530, equipped with a JetStreamSource) and negative (Agilent 6550, equipped with a dual JetStream Source) modes producing both unique and complementary spectra. Instrument parameters are as follows (positive mode) Gas Temp 325°C, Gas Flow 8 l/min, Nebulizer 35 psig, Sheath Gas 350°C, Sheath Gas Flow 11, Capillary Voltage 3500 V, Nozzle Voltage 1000V, Fragmentor 120V, Skimmer 65V. Data (both profile and centroid) are collected at a rate of 2 scans per second. In negative ion mode, Gas Temp 200°C, Gas Flow 14 l/min, Fragmentor 175V, with the other parameters identical to positive ion mode. For the 6530 QTOF, a reference solution generating ions of 121.050 and 922.007 m/z in positive mode and 119.036 and 966.0007 m/z in negative mode, and these are used for continuous mass correction. For the 6550, the reference solution is introduced via a dual spray ESI, with the same ions and continuous mass correction |
| Ion Mode: | NEGATIVE |
| MS ID: | MS004332 |
| Analysis ID: | AN004586 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Metabolon (LC/MS Pos early) |
| Ion Mode: | POSITIVE |
