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MB Sample ID: SA307543

Local Sample ID:c26_8
Subject ID:SU002952
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

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Subject:

Subject ID:SU002952
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
c26_8SA307543FL037062C26Genotype
c26_8SA307543FL037062regular chowTreatment

Collection:

Collection ID:CO002945
Collection Summary:TgLC mice were injected with Tamoxifen. Balb/c mice were injected with C26 cells. Mice were fed with a regular chow or fenofibrate diet. Liver was collected and then stored at -80 °C.
Sample Type:Liver

Treatment:

Treatment ID:TR002961
Treatment Summary:TgL and TgLC mice were injected with Tamoxifen. Balb/c mice were injected with C26 cells. Mice were fed with a regular chow or fenofibrate diet.

Sample Preparation:

Sampleprep ID:SP002958
Sampleprep Summary:For lipidomic metabolites extraction, 10 mg homogenized tissues were mixed with 120 μL methanol and 400 μL MTBE. After shaking for 6 minutes at 4 °C, 100 μL H2O was added and followed by centrifugation for 2 minutes. Supernatants were dried down for further lipidomics analysis.

Combined analysis:

Analysis ID AN004651
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE
Units Ion counts

Chromatography:

Chromatography ID:CH003501
Chromatography Summary:Lipidomics method
Instrument Name:Thermo Vanquish
Column Name:Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)
Column Temperature:55C
Flow Gradient:0 min, 25% B; 2 min, 25% B; 5.5 min, 65% B; 12.5 min, 100% B; 19.5 min, 100% B; 20.0 min, 25% B; 30 min, 25% B
Flow Rate:0.2mL/min
Solvent A:90% water/10% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate
Solvent B:75% isopropanol/25% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS004398
Analysis ID:AN004651
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Positive Ionization mode
Ion Mode:POSITIVE
Capillary Temperature:300
Ion Source Temperature:360
Spray Voltage:3500
Automatic Gain Control:3000000
Acquisition Parameters File:LCMS_Pos_RT_30min_55C_LongElution
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