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MB Sample ID: SA308217
| Local Sample ID: | WT_ddMS2 |
| Subject ID: | SU002955 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002955 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| WT_ddMS2 | SA308217 | FL037077 | TgL | Genotype |
Collection:
| Collection ID: | CO002948 |
| Collection Summary: | Whole blood was collected and centrifuged at 9000 rpm for 10 minutes. The supernatant is serum. Serum was then stored at -80 °C. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR002964 |
| Treatment Summary: | TgL and TgLC mice were injected with Tamoxifen under the fed and fasted conditions. |
Sample Preparation:
| Sampleprep ID: | SP002961 |
| Sampleprep Summary: | For lipidomic metabolites extraction, 10 μL serum were mixed with 75 μL methanol and 250 μL Methyl tert-butyl ether (MTBE). After shaking for 6 minutes at 4 °C, 62 μL H2O was added and followed by centrifugation for 2 minutes. Supernatants were dried down for further analysis. |
Combined analysis:
| Analysis ID | AN004658 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish Horizon |
| Column | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE |
| Units | Ion counts |
Chromatography:
| Chromatography ID: | CH003507 |
| Chromatography Summary: | Lipidomics method (Vanquish Horizon) |
| Instrument Name: | Thermo Vanquish Horizon |
| Column Name: | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) |
| Column Temperature: | 55C |
| Flow Gradient: | 0 min, 25% B; 2 min, 25% B; 5.5 min, 65% B; 12.5 min, 100% B; 19.5 min, 100% B; 20.0 min, 25% B; 30 min, 25% B |
| Flow Rate: | 0.2mL/min |
| Solvent A: | 90% water/10% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate |
| Solvent B: | 75% isopropanol/25% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004405 |
| Analysis ID: | AN004658 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Positive Ionization mode with ddMS2 |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 300 |
| Ion Source Temperature: | 360 |
| Spray Voltage: | 3500 |
| Automatic Gain Control: | 3000000 |
