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MB Sample ID: SA308958
| Local Sample ID: | LTEnza-3 |
| Subject ID: | SU002971 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002971 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| LTEnza-3 | SA308958 | FL037191 | 10uM Enzalutamide | Treatment |
Collection:
| Collection ID: | CO002964 |
| Collection Summary: | 16D prostate cancer cells were cultured with vehicle or Enzalutamide for >2 months. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR002980 |
| Treatment Summary: | Vehicle- and Enzalutamide-treated 16D cells were cultured in U13C-glucose containing media for 24 hours. |
Sample Preparation:
| Sampleprep ID: | SP002977 |
| Sampleprep Summary: | Cell plates were placed on ice and quickly washed with ice-cold 0.9% (w/v) NaCl. Cells were immediately treated with 500 μL of ice-cold MeOH and 200 μL water containing 1 μg of the internal standard norvaline. Cells were then scraped and placed in 1.5 mL centrifuge tubes kept on ice. Next, 500 μL of chloroform was added, after which samples were vortexed for 1 minute and then spun at 10,000g for 5 minutes at 4C. The aqueous layer was transferred to a GC/MS sample vial and dried overnight using a refrigerated CentriVap. |
Combined analysis:
| Analysis ID | AN004689 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Agilent 5977B |
| Ion Mode | POSITIVE |
| Units | abundance |
Chromatography:
| Chromatography ID: | CH003531 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| Column Temperature: | 320 |
| Flow Gradient: | N/A (GC-MS) |
| Flow Rate: | 1 ml/min |
| Solvent A: | N/A (GC-MS) |
| Solvent B: | N/A (GC-MS) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS004436 |
| Analysis ID: | AN004689 |
| Instrument Name: | Agilent 5977B |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| MS Comments: | Data was analyzed using Agilent MassHunter software. Stable isotope tracing data was corrected for natural abundance of heavy isotopes with FluxFix software using a reference set of unlabeled metabolite standards2. |
| Ion Mode: | POSITIVE |
