Return to study ST002861 main page
MB Sample ID: SA309031
| Local Sample ID: | A_137 |
| Subject ID: | SU002973 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002973 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| A_137 | SA309031 | FL037195 | high-altitude group | Treatment |
Collection:
| Collection ID: | CO002966 |
| Collection Summary: | Blood samples were obtained from both groups using evacuated blood collection tubes (Becton Dickinson MEDICAL DEVICES (Shanghai) Co., Ltd). The samples were left undisturbed for 30 minutes and then subjected to centrifugation at 5000 rpm/min for 15 minutes at a temperature of 4℃. After centrifugation the serum samples were collected and stored at -80°C before use. |
| Sample Type: | Serum |
Treatment:
| Treatment ID: | TR002982 |
| Treatment Summary: | The study enrolled two distinct groups: a plain group (PL) comprising 67 adult males from the Chengdu plain in Sichuan, and a high-altitude group (HA) comprising 163 adult males from Tibet residing at altitudes ranging from 3500m to 4500m. They were all graduated from Chengdu Medical College and enrolled in this study with the patients' consent and the sampling process was strictly consistent.The study received approval from the Ethics Committee of the Academy of Military Medicine, Chinese Academy of Military Sciences, with the approval number AF/SC-08/02.153. |
Sample Preparation:
| Sampleprep ID: | SP002979 |
| Sampleprep Summary: | The serum samples were mixed with 4-fold volumes of iced acetonitrile, which included 0.001mg/mL 4-chloro-DL-phenylalanine as an internal standard. The mixture was incubated on ice for 15 minutes to facilitate protein removal. Following centrifugation at 20,000 g at 4°C for 10 minutes, the supernatants were carefully collected into new microcentrifuge tubes. After another round of centrifugation under the same conditions, the samples were transferred to sample vials for metabolomics analysis. |
Combined analysis:
| Analysis ID | AN004691 | AN004692 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6495 QQQ | Agilent 6495 QQQ |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | counts | count |
Chromatography:
| Chromatography ID: | CH003533 |
| Chromatography Summary: | In this study, a newly developed precision-targeted metabolomics method with a UPLC-TQ/MS system (Agilent 1290 Infnity, Agilent Technologies, USA; Agilent 6495 QQQ, Agilent Technologies, USA) in a DMRM scan-mode was applied to analyze the metabolome of interest from trial samples (serum, liver tissues and stool). Briefly, the method was performed with an ACQUITY UPLC HSS T3 column (2.1 mm i.d. × 100 mm, 1.8 μm; Waters); mobile phase A and B were water and acetonitrile with 0.1% formic acid (v/v) respectively. The flow rate was at 0.3 mL/min and the column temperature was maintained at 40 ℃. The samples were placed in an auto-sampler maintained at 4 °C with a 5 μL injection volume. The optimized gradient-elution program, as follows: 0-2 min, 98% A; 2-10 min, 98-65% A; 10-12 min, 65-20% A; 12-14 min, 20-2% A; 14-30 min, 2% A. |
| Methods Filename: | Chromatography.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 40 ℃ |
| Flow Gradient: | 0-2 minutes, 98% A 2-10 minutes, 98%-65% A 10-12 minutes, 65%-20% A 12-14 minutes, 20%-2% A 14-30 minutes, 2% A. |
| Flow Rate: | 0.3 ml/min |
| Solvent A: | 100% water + 0.1% formic acid |
| Solvent B: | 100% acetonitrile + 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004438 |
| Analysis ID: | AN004691 |
| Instrument Name: | Agilent 6495 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Agilent MassHunter Workstation Data Acquisition Agilent MassHunter |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | MS.pdf |
| MS ID: | MS004439 |
| Analysis ID: | AN004692 |
| Instrument Name: | Agilent 6495 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Agilent MassHunter Workstation Data Acquisition Agilent MassHunter |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | MS.pdf |
