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MB Sample ID: SA313082
| Local Sample ID: | PP_D2 |
| Subject ID: | SU002981 |
| Subject Type: | Water sample |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002981 |
| Subject Type: | Water sample |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| PP_D2 | SA313082 | FL037408 | D | Treatment |
Collection:
| Collection ID: | CO002974 |
| Collection Summary: | Bacteria was grown in the medium containing CTAB. The water sample was collected with a syringe and filtered for storage. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR002990 |
| Treatment Summary: | Reactors are set under different conditions: composite membrane (MR) or polypropylene membrane (PP). The biofilms were treated under different conditions: A: with "160mg/L CTAB + 3g/L NH4+, low O2 pressure", B: "160mg/L CTAB + 3g/L NH4+, high O2 pressure", C:" 160mg/L CTAB, high O2 pressure", D:"400mg/L CTAB, high O2 pressure". |
Sample Preparation:
| Sampleprep ID: | SP002987 |
| Sampleprep Summary: | Liquid samples were filtered and stored in refrigerator before assay. Each sample was injected twice: 10 µL for analysis using negative ionization mode and 4 µL for analysis using positive ionization mode |
Combined analysis:
| Analysis ID | AN004702 | AN004703 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1290 | Agilent 1290 |
| Column | Waters XBridge BEH Amide (150 x 2.1 mm, 2.5um) | Waters XBridge BEH Amide (150 x 2.1 mm, 2.5um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ | Agilent 6490 QQQ |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | counts per second | counts per second |
Chromatography:
| Chromatography ID: | CH003541 |
| Chromatography Summary: | LC-MS/MS platform (Agilent 1290 UPLC-6490 QQQ-MS) |
| Instrument Name: | Agilent 1290 |
| Column Name: | Waters XBridge BEH Amide (150 x 2.1 mm, 2.5um) |
| Column Temperature: | 40 |
| Flow Gradient: | After the initial 1-min isocratic elution of 90% B, the percentage of Solvent B was gradually decreased to 40% at t = 11 min. The composition of Solvent B was maintained at 40% for 4 min (t=15 min), and then the percentage of B gradually went back to 90%, to prepare for the next injection. |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 10 mM ammonium acetate, 10 mM ammonium hydroxide in 95% H2O/5% acetonitrile |
| Solvent B: | 10 mM ammonium acetate, 10 mM ammonium hydroxide in 95% acetonitrile/5% H2O |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004448 |
| Analysis ID: | AN004702 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | LC-MS/MS |
| Ion Mode: | POSITIVE |
| MS ID: | MS004449 |
| Analysis ID: | AN004703 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | LC-MS/MS |
| Ion Mode: | NEGATIVE |
