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MB Sample ID: SA313923
| Local Sample ID: | KOFemale_1059 |
| Subject ID: | SU002985 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002985 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| KOFemale_1059 | SA313923 | FL037429 | KIKO | Genotype |
| KOFemale_1059 | SA313923 | FL037429 | KIKO_700 F | Treatment |
Collection:
| Collection ID: | CO002978 |
| Collection Summary: | Mice were anesthetized with pentobarbital. Hearts were extracted, washed in PBS and snap frozen in liquid nitrogen. |
| Sample Type: | Heart |
Treatment:
| Treatment ID: | TR002994 |
| Treatment Summary: | N/A |
Sample Preparation:
| Sampleprep ID: | SP002991 |
| Sampleprep Summary: | 15 mg of cardiac tissue was homogenized in 80% methanol (Sigma) using Tissue Tearer (BioSpec) on dry ice. Samples were incubated at -80ºC for 4 hours. Homogenates were then centrifuged at 14,000 rfc for 20 min at 4ºC. The supernatant was extracted and stored at -80ºC for mass spectroscopy (Weill Cornell Medicine Meyer Cancer Center Proteomics & Metabolomics Core Facility). Peak intensities for metabolites were screened for missing values and analyzed by using MetaboAnalyst software (version 5.0). |
Combined analysis:
| Analysis ID | AN004708 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | SeQuant ZIC-pHILIC (150 x 2.1mm, 5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | Peak intensity |
Chromatography:
| Chromatography ID: | CH003545 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | SeQuant ZIC-pHILIC (150 x 2.1mm, 5um) |
| Column Temperature: | 30 |
| Flow Gradient: | 85% to 30% acetonitrile (ACN) |
| Flow Rate: | 150 μL/min |
| Solvent A: | 0.1% NH4OH/20 mM CH3COONH4 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004454 |
| Analysis ID: | AN004708 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | MS acquisition Comments: The Q Exactive was operated in full scan, polarity-switching mode with the following parameters: the spray voltage 3.0 kV, the heated capillary temperature 300 °C, the HESI probe temperature 350 °C, the sheath gas flow 40 units, the auxiliary gas flow 15 units. MS data acquisition was performed in the m/z range of 70–1,000, with 70,000 resolution (at 200 m/z). Data processing Comments: The MS data was processed using XCalibur 4.1 (Thermo Scientific) to extract the metabolite signal intensity for relative quantitation. Metabolites were identified using an in-house library established using chemical standards. Identification required exact mass (within 5ppm) and standard retention times. Software/procedures used for feature assignments: The MS data was processed using XCalibur 4.1 (Thermo Scientific) to extract the metabolite signal intensity for relative quantitation. Metabolites were identified using an in-house library established using chemical standards. Identification required exact mass (within 5ppm) and standard retention times. |
| Ion Mode: | POSITIVE |
