Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA314134

Local Sample ID:	A47
Subject ID:	SU002986
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Subject:

Subject ID:	SU002986
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
A47	SA314134	FL037437	AAA	treatment

Collection:

Collection ID:	CO002979
Collection Summary:	Plasma samples from 100 AAA patients and 100 non-AAA controls were collected from Beijing Anzhen Hospital of Capital Medical University and Tongji Medical College of Huazhong University of Science and Technology.
Sample Type:	plasma
Storage Conditions:	-80℃

Treatment:

Treatment ID:	TR002995
Treatment Summary:	Plasma samples from 100 AAA patients and 100 non-AAA controls were collected from Beijing Anzhen Hospital of Capital Medical University and Tongji Medical College of Huazhong University of Science and Technology.

Sample Preparation:

Sampleprep ID:	SP002992
Sampleprep Summary:	Briefly, 50 μL plasma spiked with 10 µL internal standard mixture and 10 µL BHT was blended into 380 µL methanol (75%). MTBE (1 mL) was added, and the mixture was vortexed for 30 min. Phase separation was induced by adding 250 µL H₂O. After incubation for 10 min at room temperature, the samples were centrifuged at 14 000 g for 10 min. The upper phase was collected and dried using a nitrogen evaporator. Ultra-performance liquid chromatography BEH C18 columns (1.7 μm, 100 × 2.1 mm i.d.) were used for chromatographic separations.

Sampleprep ID:

SP002992

Sampleprep Summary:

Briefly, 50 μL plasma spiked with 10 µL internal standard mixture and 10 µL BHT was blended into 380 µL methanol (75%). MTBE (1 mL) was added, and the mixture was vortexed for 30 min. Phase separation was induced by adding 250 µL H₂O. After incubation for 10 min at room temperature, the samples were centrifuged at 14 000 g for 10 min. The upper phase was collected and dried using a nitrogen evaporator. Ultra-performance liquid chromatography BEH C18 columns (1.7 μm, 100 × 2.1 mm i.d.) were used for chromatographic separations.

Combined analysis:

Analysis ID	AN004709	AN004710
Analysis type	MS	MS
Chromatography type	Reversed phase	Reversed phase
Chromatography system	Waters Acquity	Waters Acquity
Column	Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)	Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
MS Type	ESI	ESI
MS instrument type	QTRAP	QTRAP
MS instrument name	ABI Sciex 5500 QTrap	ABI Sciex 5500 QTrap
Ion Mode	POSITIVE	NEGATIVE
Units	ng/mL	ng/mL

Chromatography:

Chromatography ID:	CH003546
Instrument Name:	Waters Acquity
Column Name:	Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
Column Temperature:	50
Flow Gradient:	0.25mL/min：0-3min：25%A：75%B，3-17min：99%A:1%B, 17-20min：25%A:75%B
Flow Rate:	0.25mL/min
Solvent A:	90% isopropanol/10% acetonitrile; 5mM ammonium acetate
Solvent B:	60% acetonitrile
Chromatography Type:	Reversed phase

MS:

MS ID:	MS004455
Analysis ID:	AN004709
Instrument Name:	ABI Sciex 5500 QTrap
Instrument Type:	QTRAP
MS Type:	ESI
MS Comments:	Raw data were processed using MultiQuant 3.0.3. Metabolite levels were calculated as the ratio of their concentrations relative to a standard sample. Quantifing the targeted metabolites by area under the curve used expected retention time.
Ion Mode:	POSITIVE

MS ID:	MS004456
Analysis ID:	AN004710
Instrument Name:	ABI Sciex 5500 QTrap
Instrument Type:	QTRAP
MS Type:	ESI
MS Comments:	Raw data were processed using MultiQuant 3.0.3. Metabolite levels were calculated as the ratio of their concentrations relative to a standard sample. Quantifing the targeted metabolites by area under the curve used expected retention time.
Ion Mode:	NEGATIVE