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MB Sample ID: SA314466

Local Sample ID:LAT1-4F2hc_WT_delipidated_1
Subject ID:SU002988
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Cell Strain Details:HEK293F

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Subject:

Subject ID:SU002988
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Cell Strain Details:HEK293F

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
LAT1-4F2hc_WT_delipidated_1SA314466FL037442DelipidatedTreatment

Collection:

Collection ID:CO002981
Collection Summary:The LAT1-4F2hc complex was buffer-exchanged into 1M ammonium acetate, pH 7.0 with 2 mM OGNG and incubated with 1 µg trypsin overnight at 37 °C. The digested peptide/lipid mixture was dried using a SpeedVac vacuum concentrator (Thermo Fisher Scientific)
Sample Type:HEK cells

Treatment:

Treatment ID:TR002997
Treatment Summary:The LAT1-4F2hc complex was buffer-exchanged into 1M ammonium acetate (pH 7.0) with 20 mM OGNG using a 100 kD MWCO centrifugal filter (Amicon Ultra-0.5 ml, Millipore) and incubated for 4 h at room temperature with gentle mixing. Excess lipomicelles and detergents were removed by buffer-exchanging into 1 M ammonium acetate with 2 mM OGNG using the same filter.

Sample Preparation:

Sampleprep ID:SP002994
Sampleprep Summary:The lipid mixture was reconstituted with 70% mobile phase A (acetonitrile/H2O: 60/40, 10 mM ammonium formate and 0.1% formic acid) and 30% mobile phase B (isopropanol/acetonitrile: 90/10, 10 mM ammonium formate and 0.1% formic acid) for the following LC-MS/MS analysis.

Combined analysis:

Analysis ID AN004712
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Dionex Ultimate 3000
Column Thermo Acclaim PepMap 100 C18 (150 x 0.007 mm, 3um, 100A)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo LTQ XL
Ion Mode NEGATIVE
Units Peak area

Chromatography:

Chromatography ID:CH003548
Chromatography Summary:Column information: https://www.thermofisher.com/order/catalog/product/164568
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Thermo Acclaim PepMap 100 C18 (150 x 0.007 mm, 3um, 100A)
Column Temperature:40
Flow Gradient:30%-99%
Flow Rate:300 nl/min
Solvent A:60% acetonitrile/40% water; 10 mM ammonium formate; 0.1% formic acid
Solvent B:90% isopropanol/10% acetonitrile; 10 mM ammonium formate; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004458
Analysis ID:AN004712
Instrument Name:Thermo LTQ XL
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
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