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MB Sample ID: SA317744

Local Sample ID:s_AD_FA_36
Subject ID:SU003037
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Not applicable
Human Race:Pacific asian

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Subject:

Subject ID:SU003037
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Not applicable
Human Race:Pacific asian

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
s_AD_FA_36SA317744FL037818AD_FAFactor

Collection:

Collection ID:CO003030
Collection Summary:The study population consisted of 158 six-month-old infants (46 healthy infants, 30 only AD group, and 82 with combined AD and FA) involved in the Cohort for Childhood Origin of Asthma and Allergic Diseases (COCOA) (reference: BMC Pulm Med 2014, 14:109.)
Sample Type:Feces

Treatment:

Treatment ID:TR003046
Treatment Summary:no treatment

Sample Preparation:

Sampleprep ID:SP003043
Sampleprep Summary:~40 mg of feces was used and lipids were extracted by Bligh and Dyer method after adding internal standard solutions (50 μl of 100 nM C18 ceramide-d7 and C18:1 Sphingomyelin-d9 and 200 μl of 1 μM 1,3-19:0-d5). Organic solutions containing the lipids were dried using a vacuum centrifuge and stored at –20°C until LC-MS/MS analysis. The dried matter was reconstituted with methanol and injected into the LC-MS/MS system.

Combined analysis:

Analysis ID AN004794 AN004795
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity
Column Agilent Pursuit 5 C18 (150 x 2.0 mm, 5um) Agilent Pursuit 5 C18 (150 x 2.0 mm, 5um)
MS Type ESI ESI
MS instrument type QTRAP QTRAP
MS instrument name ABI Sciex 5500 QTrap ABI Sciex 5500 QTrap
Ion Mode POSITIVE POSITIVE
Units pmol/mg pmol/mg

Chromatography:

Chromatography ID:CH003625
Chromatography Summary:Ceramides, sphingomyelines, sphinganine, sphingosine
Instrument Name:Agilent 1290 Infinity
Column Name:Agilent Pursuit 5 C18 (150 x 2.0 mm, 5um)
Column Temperature:35
Flow Gradient:50 % of B at 0 min, 50 % of B for 5 min, 50-70 % of B for 3 min, 70 % of B for 7 min, 70-90 % of B for 7 min, 90 % of B for 3 min, 90-50 % of B for 0.1 min, 50 % of B for 4.9 min
Flow Rate:200 uL/min
Solvent A:5 mM ammonium formate/MeOH/tetrahydrofuran (500/200/300, v/v/v)
Solvent B:5 mM ammonium formate/MeOH/ tetrahydrofuran (100/200/700, v/v/v)
Chromatography Type:Reversed phase
  
Chromatography ID:CH003626
Chromatography Summary:diacylglycerols
Instrument Name:Agilent 1290 Infinity
Column Name:Agilent Pursuit 5 C18 (150 x 2.0 mm, 5um)
Column Temperature:35
Flow Gradient:90 % of B for 0 min, 90 % of B for 6 min, 90 to 95 % of B for 0.6 min, 95 % of B for 3.4 min, 95 to 90 % of B for 0.1 min, and 90 % of B for 1.9 min
Flow Rate:200 uL/min
Solvent A:5 mM ammonium formate/MeOH/tetrahydrofuran (500/200/300, v/v/v)
Solvent B:5 mM ammonium formate/MeOH/ tetrahydrofuran (100/200/700, v/v/v)
Chromatography Type:Reversed phase

MS:

MS ID:MS004540
Analysis ID:AN004794
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:Multiple reaction monitoring (MRM) was performed in the positive ion mode and the extracted ion chromatogram corresponding to the specific transition for each lipid was used for quantification. The calibration range for each lipid was 0.1-1000 nM (r2 ≥ 0.99). Data analysis was performed by using Analyst 1.5.2 software.
Ion Mode:POSITIVE
Acquisition Parameters File:MS_metadata.docx
  
MS ID:MS004541
Analysis ID:AN004795
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:Multiple reaction monitoring (MRM) was performed in the positive ion mode and the extracted ion chromatogram corresponding to the specific transition for each lipid was used for quantification. The calibration range for each lipid was 0.1-1000 nM (r2 ≥ 0.99). Data analysis was performed by using Analyst 1.5.2 software.
Ion Mode:POSITIVE
Acquisition Parameters File:MS_metadata.docx
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