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MB Sample ID: SA317882
| Local Sample ID: | sample_13 |
| Subject ID: | SU003038 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 10-week |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003038 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 10-week |
| Gender: | Male |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| sample_13 | SA317882 | FL037821 | wild-type | Genotype |
| sample_13 | SA317882 | FL037821 | fed | Treatment |
Collection:
| Collection ID: | CO003031 |
| Collection Summary: | Mice were sacrificed and liver samples were dissected out and snap-frozen in liquid nitrogen. |
| Sample Type: | Liver |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003047 |
| Treatment Summary: | We performed retro-orbital injection of P28 old Pptc7-flox (GemPharmatech, T011006) mice with 1×10^12 vector genome of the empty vector or Cre AAV8 virus. 42 days post AAV injection, mice were subjected to fasting or left fed as control group respectively. Fasting was done for 18 hours from the beginning of the dark cycle at 7.p.m. to 1.p.m. next day. |
Sample Preparation:
| Sampleprep ID: | SP003044 |
| Sampleprep Summary: | Mouse livers were dissected out and snap-frozen in liquid nitrogen. Liver samples were cryo-homogenized on dry ice and 30 mg of homogenized tissue powders were weighed and extracted with 600 μL ice-cold acetonitrile/methanol/water (4/4/2) for 30 min. After centrifugation at 20,000 g for 15 min, supernatant was transferred to a new tube and dried by a Speedvac vacuum concentrator at 30°C. Dried samples were stored at -80°C and subjected to LC-MS analysis within 24 hours. |
Combined analysis:
| Analysis ID | AN004796 | AN004797 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Thermo Vanquish UHPLC | Thermo Vanquish UHPLC |
| Column | Merck ZIC-HILIC (2.1x100 mm, 3.5 μm) | Merck ZIC-HILIC (2.1x100 mm, 3.5 μm) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF-X Orbitrap | Thermo Q Exactive HF-X Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | count | count |
Chromatography:
| Chromatography ID: | CH003627 |
| Instrument Name: | Thermo Vanquish UHPLC |
| Column Name: | Merck ZIC-HILIC (2.1x100 mm, 3.5 μm) |
| Column Temperature: | 40 |
| Flow Gradient: | 0-5 min, 99% B; 5-20 min, 99-20% B; 20-21 min, 20-99% B; 21-25 min, 99% B. |
| Flow Rate: | 0.5 mL/min |
| Solvent A: | 5% acetonitrile/95% water; 10 mM ammonium acetate |
| Solvent B: | 95% acetonitrile/5% water; 10 mM ammonium acetate |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004542 |
| Analysis ID: | AN004796 |
| Instrument Name: | Thermo Q Exactive HF-X Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | POSITIVE |
| MS ID: | MS004543 |
| Analysis ID: | AN004797 |
| Instrument Name: | Thermo Q Exactive HF-X Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | NEGATIVE |
