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MB Sample ID: SA317902
| Local Sample ID: | CP_2c_5 |
| Subject ID: | SU003039 |
| Subject Type: | Cultured cells |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003039 |
| Subject Type: | Cultured cells |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| CP_2c_5 | SA317902 | FL037833 | 2c HA control-4 | treatment |
Collection:
| Collection ID: | CO003032 |
| Collection Summary: | Highly synchronised parasites were harvested by magnet purification (Ribaut et al., 2008). 140 µL of 100% methanol was added to 5 x 107 parasites to extract metabolites. Samples were incubated at 4 °C for 1 h on a vortex shaker (Select Bioproducts). Samples were centrifuged at 21,000 x g for 5 min and the particle-free supernatant stored at – 80 °C until analysis. |
| Sample Type: | Blood (whole) |
Treatment:
| Treatment ID: | TR003048 |
| Treatment Summary: | untargeted metabolomics was performed on PfPK8-KO clone 10g2 and clone 11c, and on PfPK8-HA clone 2c (control). |
Sample Preparation:
| Sampleprep ID: | SP003045 |
| Sampleprep Summary: | Highly synchronised parasites were harvested by magnet purification (Ribaut et al., 2008). 140 µL of 100% methanol was added to 5 x 107 parasites to extract metabolites. Samples were incubated at 4 °C for 1 h on a vortex shaker (Select Bioproducts). Samples were centrifuged at 21,000 x g for 5 min and the particle-free supernatant stored at – 80 °C until analysis. |
Combined analysis:
| Analysis ID | AN004798 | AN004799 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | QExactive | QExactive |
| Column | Merck SeQuant ZIC-pHILIC (150 x 4.6mm,5um) | Merck SeQuant ZIC-pHILIC (150 x 4.6mm,5um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak height | peak height |
Chromatography:
| Chromatography ID: | CH003628 |
| Instrument Name: | QExactive |
| Column Name: | Merck SeQuant ZIC-pHILIC (150 x 4.6mm,5um) |
| Column Temperature: | 25 |
| Flow Gradient: | 0% B to 50% B over 15 min, then to 5% B at 18 min until 21 min, increasing to 80% B at 24 min until 32 min. |
| Flow Rate: | 0.3 ml/min |
| Solvent A: | 100% water; 20 mM ammonium carbonate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004544 |
| Analysis ID: | AN004798 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The MS was fitted with a heated electrospray source that switched between positive and negative ionisation modes. The MS operated at a mass resolution of 35,000 from m/z 85 to 1,050. |
| Ion Mode: | POSITIVE |
| MS ID: | MS004545 |
| Analysis ID: | AN004799 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The MS was fitted with a heated electrospray source that switched between positive and negative ionisation modes. The MS operated at a mass resolution of 35,000 from m/z 85 to 1,050. |
| Ion Mode: | NEGATIVE |
