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MB Sample ID: SA317906
| Local Sample ID: | C14 |
| Subject ID: | SU003040 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003040 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| C14 | SA317906 | FL037835 | RecoveryHeLa | Factor |
Collection:
| Collection ID: | CO003033 |
| Collection Summary: | Care was taken to quench cells quickly to minimize oxidation and degradation. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR003049 |
| Treatment Summary: | HeLa cells of shControl or shOGA were treated with no stress, stress by 1 M sorbitol for 1 hr, or recovery from stress for 2 hr. |
Sample Preparation:
| Sampleprep ID: | SP003046 |
| Sampleprep Summary: | Briefly, equal confluency of cells in 6-well plate with 3 biological replicates were rinsed by 1 mL HPLC-grade water rapidly and immediately quenched by directly added by 1 mL liquid nitrogen to the plate. Then, 1 mL ice-cold 90% MC buffer (MC, 9: 1 MeOH: CHCl3. 90% MC, 9 : 1 MC: water) with 5 µM methionine sulfone as internal signal was added to each well of plate and the cells were scraped and transferred to1.5-mL tubes. Extracts were centrifuged at 4 ℃ for 5 min at 16,000 xg and the supernatants were transferred to new clean 1.5-mL tubes and equal volume of each sample was pooled for the QC sample. Finally, the extracts were dried using vacuum concentrator system and store at -80 ℃ until analysis. For targeted analysis, the dried extracts were resuspended by 100 µL 60% acetonitrile and centrifuged at 4 ℃ for 15 min at 15,000 xg after 30 min incubation at 4 ℃. Only the supernatant was transferred to autosampler vials for MS analysis. |
Combined analysis:
| Analysis ID | AN004800 | AN004801 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Shimadzu 20AD | Shimadzu 20AD |
| Column | Merck SeQuant ZIC-HILIC (150 x 2.1mm,5um) | Merck SeQuant ZIC-HILIC (150 x 2.1mm,5um) |
| MS Type | ESI | ESI |
| MS instrument type | QTRAP | QTRAP |
| MS instrument name | ABI Sciex Triple Quad 5500+ | ABI Sciex Triple Quad 5500+ |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak intensity | Peak intensity |
Chromatography:
| Chromatography ID: | CH003629 |
| Instrument Name: | Shimadzu 20AD |
| Column Name: | Merck SeQuant ZIC-HILIC (150 x 2.1mm,5um) |
| Column Temperature: | 45 |
| Flow Gradient: | 0.01 min 80% B, 20 min 20% B, 20.5 min 80% B, 24 min 80% B. |
| Flow Rate: | 0.15 mL/min |
| Solvent A: | 100% water, 20 mM ammonium carbonate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004546 |
| Analysis ID: | AN004800 |
| Instrument Name: | ABI Sciex Triple Quad 5500+ |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | The raw data were extracted with the software Analyst v1.7.2 and OS v1.7. |
| Ion Mode: | POSITIVE |
| MS ID: | MS004547 |
| Analysis ID: | AN004801 |
| Instrument Name: | ABI Sciex Triple Quad 5500+ |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | The raw data were extracted with the software Analyst v1.7.2 and OS v1.7. |
| Ion Mode: | NEGATIVE |
