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MB Sample ID: SA318136

Local Sample ID:bd_s8
Subject ID:SU003044
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:20 wks
Gender:Male
Animal Animal Supplier:Jackson Lab

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Subject:

Subject ID:SU003044
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:20 wks
Gender:Male
Animal Animal Supplier:Jackson Lab

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
bd_s8SA318136FL037862bloodSample
bd_s8SA318136FL037862SedentaryTreatment

Collection:

Collection ID:CO003037
Collection Summary:Mice were randomized into two groups, sedentary control and running experimental. Mice were housed with free access to a wireless running wheel (Med Associates). For the sedentary group, the wheels were locked in the experiment. After 42 days, blood (130 uL), feces, hippocampus and brainstem were collected and stored at -80°C.
Sample Type:blood, feces, hippocampus, brainstem
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003053
Treatment Summary:To evaluate our hypothesis that running exercise reshapes gut microbiota diversity that balances TRP metabolism in the gut, we used an established model of voluntary running exercise in 20-week-old male mice (C57BL/6J, Jackson Lab) housed individually in a standard cage in temperature-controlled (21°C) quarters with a 12-h light/12-h dark cycle. Animals were given water and food (Purina Chow) ad libitum as previously described (Chorna et al., 2013). Briefly, mice were randomized into two groups, sedentary control (n=12) and running experimental (n=12), housed with free access to a wireless running wheel (Med Associates) for six weeks. For the sedentary group, the wheels were locked in the experiment. Therefore, this group of mice could not perform running exercises. Exercise activities of the running group were recorded for each animal for the investigation to ensure that each mouse was physically active. The recording was conducted using an automatic counter and Med Associates software.

Sample Preparation:

Sampleprep ID:SP003050
Sampleprep Summary:Metabolites were extracted from feces, blood, brainstem and hippocampus. See Sample_preparation.pdf.
Sampleprep Protocol Filename:Sample_preparation.pdf
Processing Storage Conditions:Described in summary
Extract Storage:Described in summary

Combined analysis:

Analysis ID AN004809
Analysis type MS
Chromatography type GC
Chromatography system Shimadzu GCMS/MS-TQ8050
Column Shimadzu SH-Rxi-5ms (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type Triple quadrupole
MS instrument name Shimadzu GCMS/MS-TQ8050
Ion Mode POSITIVE
Units intensity

Chromatography:

Chromatography ID:CH003634
Chromatography Summary:1 μL of each sample was injected in the GC-MS. Injection mode – split (15:1). The injection port temperature was set at 280°C, and helium was used as the carrier gas at a constant linear velocity of 39 cm/sec. Metabolites were separated using a GC temperature ramping program. The GC oven was programmed from 100°C to 280°C at a rate of 4°C/min.
Methods Filename:Chromatography.pdf
Instrument Name:Shimadzu GCMS/MS-TQ8050
Column Name:Shimadzu SH-Rxi-5ms (30m x 0.25mm,0.25um)
Column Temperature:280°C
Flow Gradient:NA
Flow Rate:4°C/min
Solvent A:NA
Solvent B:NA
Chromatography Type:GC

MS:

MS ID:MS004555
Analysis ID:AN004809
Instrument Name:Shimadzu GCMS/MS-TQ8050
Instrument Type:Triple quadrupole
MS Type:EI
MS Comments:MS acquisition: Metabolites were detected by setting the ion source filament energy to 70 eV, the ion source temperature - 200°C, and the scan range (mass-to-charge (m/z) 35 – 600 Da. Software/procedures used for feature assignments: Labsolution Shimadzu
Ion Mode:POSITIVE
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