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MB Sample ID: SA318421
Local Sample ID: | C9 |
Subject ID: | SU003049 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003049 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
C9 | SA318421 | FL037883 | StressHeLa | Factor |
Collection:
Collection ID: | CO003042 |
Collection Summary: | Care was taken to quench cells quickly to minimize oxidation and degradation. |
Sample Type: | Cultured cells |
Treatment:
Treatment ID: | TR003058 |
Treatment Summary: | HeLa cells of shControl or shOGA were treated with no stress, stress by 1 M sorbitol for 1 hr, or recovery from stress for 2 hr. |
Sample Preparation:
Sampleprep ID: | SP003055 |
Sampleprep Summary: | Briefly, equal confluency of cells in 6-well plate with 3 biological replicates were rinsed by 1 mL HPLC-grade water rapidly and immediately quenched by directly added by 1 mL liquid nitrogen to the plate. Then, 1 mL ice-cold 90% MC buffer (MC, 9: 1 MeOH: CHCl3. 90% MC, 9 : 1 MC: water) with 5 µM methionine sulfone as internal signal was added to each well of plate and the cells were scraped and transferred to1.5-mL tubes. Extracts were centrifuged at 4 ℃ for 5 min at 16,000 xg and the supernatants were transferred to new clean 1.5-mL tubes and equal volume of each sample was pooled for the QC sample. Finally, the extracts were dried using vacuum concentrator system and store at -80 ℃ until analysis. For targeted analysis, the dried extracts were resuspended by 100 µL 60% acetonitrile and centrifuged at 4 ℃ for 15 min at 15,000 xg after 30 min incubation at 4 ℃. Only the supernatant was transferred to autosampler vials for MS analysis. |
Combined analysis:
Analysis ID | AN004815 | AN004816 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Shimadzu 20AD | Shimadzu 20AD |
Column | Merck SeQuant ZIC-HILIC (150 x 2.1mm,5um) | Merck SeQuant ZIC-HILIC (150 x 2.1mm,5um) |
MS Type | ESI | ESI |
MS instrument type | QTRAP | QTRAP |
MS instrument name | ABI Sciex Triple Quad 5500+ | ABI Sciex Triple Quad 5500+ |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak intensity | Peak intensity |
Chromatography:
Chromatography ID: | CH003639 |
Instrument Name: | Shimadzu 20AD |
Column Name: | Merck SeQuant ZIC-HILIC (150 x 2.1mm,5um) |
Column Temperature: | 45 |
Flow Gradient: | 0.01 min 80% B, 20 min 20% B, 20.5 min 80% B, 24 min 80% B. |
Flow Rate: | 0.15 mL/min |
Solvent A: | 100% water; 20 mM ammonium carbonate |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |
MS:
MS ID: | MS004561 |
Analysis ID: | AN004815 |
Instrument Name: | ABI Sciex Triple Quad 5500+ |
Instrument Type: | QTRAP |
MS Type: | ESI |
MS Comments: | The raw data were extracted with the software Analyst v1.7.2 and OS v1.7. This is a continuation of study ST002927 where a different set precusror/product ions were chosen for MS. |
Ion Mode: | POSITIVE |
MS ID: | MS004562 |
Analysis ID: | AN004816 |
Instrument Name: | ABI Sciex Triple Quad 5500+ |
Instrument Type: | QTRAP |
MS Type: | ESI |
MS Comments: | The raw data were extracted with the software Analyst v1.7.2 and OS v1.7. This is a continuation of study ST002927 where a different set precursor/product ions were chosen for MS. |
Ion Mode: | NEGATIVE |