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MB Sample ID: SA320272
| Local Sample ID: | s118-2 |
| Subject ID: | SU003056 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003056 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| s118-2 | SA320272 | FL037944 | 2 | time_weeks |
| s118-2 | SA320272 | FL037944 | control | intervention |
Collection:
| Collection ID: | CO003049 |
| Collection Summary: | Fecal samples were collected longitudinally at 2-week intervals during the 8-week study. Samples were kept on ice immediately after collection and snap-frozen, then stored in -80 freezers until processing. |
| Sample Type: | Feces |
Treatment:
| Treatment ID: | TR003065 |
| Treatment Summary: | Participants were randomly allocated to intervention arms to receive either 24 mg or a rice protein placebo capsule daily for 8 weeks. Clinic check-in and sample collections occurred at baseline and 2-week intervals until study close-out (week 8). |
Sample Preparation:
| Sampleprep ID: | SP003062 |
| Sampleprep Summary: | Fecal samples were dried using vacuum desiccator and 70% methanol:water (1:4 w/v) was added for fecal homogenate. Samples were centrifuged and supernatant was collected. 10 uL of fecal supernatant, 10 uL of cholic acid-D4 internal standard (1 ug/mL in ethanol) and 80 uL of 50% methanol:water were mixed and added to a autosampler vial. |
Combined analysis:
| Analysis ID | AN004828 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu 20AD |
| Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 4000 QTrap |
| Ion Mode | NEGATIVE |
| Units | ng/g |
Chromatography:
| Chromatography ID: | CH003648 |
| Instrument Name: | Shimadzu 20AD |
| Column Name: | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0 to 1 min, 35% B; 10 min, 60% B; 10.5 min, 99% B; 13 min, 99% B; 13.5 to 17 min, 35% B |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | water with 0.1% formic acid (v/v) |
| Solvent B: | acetonitrile with 0.1% formic acid (v/v) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004574 |
| Analysis ID: | AN004828 |
| Instrument Name: | ABI Sciex 4000 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Metabolites were confirmed by matching m/z values, retention times and product ions of SRM transitions with those of reference standards |
| Ion Mode: | NEGATIVE |
