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MB Sample ID: SA322958
| Local Sample ID: | MB-A2 |
| Subject ID: | SU003079 |
| Subject Type: | Plant |
| Subject Species: | Corydalis yanhusuo |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003079 |
| Subject Type: | Plant |
| Subject Species: | Corydalis yanhusuo |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| MB-A2 | SA322958 | FL038513 | mother-bulb expansion period | Treatment |
Collection:
| Collection ID: | CO003072 |
| Collection Summary: | In this study, materials of C. yanhusuo bulbs were cultivated in the field, which was proposed and identified by Professor Da-xia Chen. On April 6th (expansion period) and April 26th (maturity period), samples of the "mother bulb" and "son bulb" of C. yanhusuo were collected. After washing, the samples were immediately placed into liquid nitrogen for quick freezing and then transferred to an ultralow temperature refrigerator for storage |
| Sample Type: | Plant |
Treatment:
| Treatment ID: | TR003088 |
| Treatment Summary: | Different suborgan parts and development periods of C. yanhusuo bulb. MB-A: mother-bulb expansion period, SB-A: son-bulb expansion period, MB-C: mother-bulb maturity period, SB-C: son-bulb maturity period. |
Sample Preparation:
| Sampleprep ID: | SP003085 |
| Sampleprep Summary: | After vacuum freeze-drying, the biological sample was crushed at 30 Hz for 1.5 minutes using a mixer (MM 400, Retsch) with zirconia beads. One hundred milligrams of lyophilized powder was accurately weighed, dissolved in 1.2 ml of 70% methanol solution, rotated every 30 minutes for 30 seconds, repeated 6 times, and placed in a refrigerator at 4 °C for 12 h. Finally, after centrifuging the solution at 12 000 rpm for 10 minutes, the extract was filtered (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China, http://www.anpel.com.cn/) to perform UPLC‒MS/MS analysis. |
Combined analysis:
| Analysis ID | AN004873 | AN004874 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | SHIMADZU Nexera X2 | SHIMADZU Nexera X2 |
| Column | Agilent ZORBAX RRHD SB-C18 (100 x 2.1mm,1.8um) | Agilent ZORBAX RRHD SB-C18 (100 x 2.1mm,1.8um) |
| MS Type | ESI | APCI |
| MS instrument type | QTRAP | QTRAP |
| MS instrument name | ABI Sciex API 4000 QTrap | ABI Sciex API 4000 QTrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH003677 |
| Instrument Name: | SHIMADZU Nexera X2 |
| Column Name: | Agilent ZORBAX RRHD SB-C18 (100 x 2.1mm,1.8um) |
| Column Temperature: | 40°C |
| Flow Gradient: | 95% A, 5% B. Within 9 min, a linear gradient to 5% A, 95% B was programmed, and a composition of 5% A, 95% B was kept for 1 min. Subsequently, a composition of 95% A, 5.0% B was adjusted within 1.1 min and kept for 2.9 min |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | pure water with 0.1% formic acid |
| Solvent B: | acetonitrile with 0.1% formic acid |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004617 |
| Analysis ID: | AN004873 |
| Instrument Name: | ABI Sciex API 4000 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), AB4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature 550°C; ion spray voltage (IS) 5500 V (positive ion mode)/-4500 V (negative ion mode); ion source gas I (GSI), gas II(GSII), curtain gas (CUR) were set at 50, 60, and 25.0 psi, respectively; the collision-activated dissociation(CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to medium. DP and CE for individual MRM transitions was done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period. |
| Ion Mode: | POSITIVE |
| MS ID: | MS004618 |
| Analysis ID: | AN004874 |
| Instrument Name: | ABI Sciex API 4000 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | APCI |
| MS Comments: | LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), AB4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature 550°C; ion spray voltage (IS) 5500 V (positive ion mode)/-4500 V (negative ion mode); ion source gas I (GSI), gas II(GSII), curtain gas (CUR) were set at 50, 60, and 25.0 psi, respectively; the collision-activated dissociation(CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to medium. DP and CE for individual MRM transitions was done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period. |
| Ion Mode: | NEGATIVE |
