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MB Sample ID: SA326849
| Local Sample ID: | EF_eWAT_neg_2 |
| Subject ID: | SU003118 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 12 weeks |
| Weight Or Weight Range: | 25-30g |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003118 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 12 weeks |
| Weight Or Weight Range: | 25-30g |
| Gender: | Male |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| EF_eWAT_neg_2 | SA326849 | FL038829 | eWAT | Tissue |
Collection:
| Collection ID: | CO003111 |
| Collection Summary: | Animals were sacrificed immediately by cervical dislocation and tissues were rapidly extracted. Tissues were subjected to centrifugation (10 min, 800 g, 4°C) following placement in a 20 μm nylon mesh filter (EMD Millipore). |
| Sample Type: | Extracellular fluid |
Treatment:
| Treatment ID: | TR003127 |
| Treatment Summary: | All mice were housed under a 12 h – 12 h light/dark cycle. Room-temperature mice were housed at 23˚C in ventilated cages with an ACH of 25. Mice were fed a standard diet (Lab Diet 5008) and had free access to food and water. |
Sample Preparation:
| Sampleprep ID: | SP003124 |
| Sampleprep Summary: | Animals were sacrificed immediately by cervical dislocation. Tissues were subjected to centrifugation (10 min, 800 g, 4°C) following placement in a 20 μm nylon mesh filter (EMD Millipore). Metabolites were extracted by adding extraction buffer at a ratio of 1:100 interstitial fluid to methanol. Samples were then centrifuged twice (5 min, 10,000 g, 4°C) and supernatant was collected. |
Combined analysis:
| Analysis ID | AN004935 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo orbitrap exploris 240 |
| Ion Mode | NEGATIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003724 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| Column Temperature: | 25 °C |
| Flow Gradient: | The linear gradient eluted from 95% B (0.0–1 min), 95% B to 65% B (1–7.0 min), 65% B to 40% B (7.0–8.0 min), 40% B (8.0–9.0 min), 40% B to 95% B (9.0–9.1 min), then stayed at 95% B for 5.9 min. |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 25mM ammonium acetate; 25mM ammonium hydroxide |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004678 |
| Analysis ID: | AN004935 |
| Instrument Name: | Thermo orbitrap exploris 240 |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | ESI source parameters were set as follows: spray voltage, 3500 V or −2800 V, in positive or negative modes, respectively; vaporizer temperature, 350 °C; sheath gas, 50 arb; aux gas, 10 arb; ion transfer tube temperature, 325 °C. The full scan was set as: orbitrap resolution, 60,000; maximum injection time, 100 ms; scan range, 70–1050 Da. The ddMS2 scan was set as: orbitrap resolution, 30,000; maximum injection time, 60 ms; top N setting, 6; isolation width, 1.0 m/z; HCD collision energy (%), 30; Dynamic exclusion mode was set as auto. The data was analyzed by Compound Discoverer 3.3. |
| Ion Mode: | NEGATIVE |
