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MB Sample ID: SA330691
| Local Sample ID: | 36 |
| Subject ID: | SU003161 |
| Subject Type: | Mammal |
| Subject Species: | Sus scrofa |
| Taxonomy ID: | 9823 |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003161 |
| Subject Type: | Mammal |
| Subject Species: | Sus scrofa |
| Taxonomy ID: | 9823 |
| Gender: | Male |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 36 | SA330691 | FL039133 | SHAM | Pig_Model |
| 36 | SA330691 | FL039133 | 180 | Time_from_End_of_Shock_min |
Collection:
| Collection ID: | CO003154 |
| Collection Summary: | Healthy adolescent, male Yorkshire swine (average weight 50.0 ± 2.7 kg) were acclimated in the animal facilities for a minimum of 3 days and examined by a veterinarian on the day of experimentation. In brief, on the day of surgery, swine anesthesia was induced via intramuscular injection of ketamine (20.0 mg/kg), xylazine (2.0 mg/kg), and acepromazine (0.2 mg/kg) followed by intubation under direct laryngoscopy. Continuous infusion anesthesia was maintained through a superficial ear vein using propofol (3 mg/kg/h) and fentanyl (3 mcg/kg/h). Lactated Ringer’s solution was administered at a rate of 10 mL/kg/h to overcome insensible losses. Mahurkar catheters (12 Fr) were placed in the femoral arteries and veins for vascular access. |
| Collection Protocol ID: | Animal Care and Use Committee approved this animal study under protocol #1050 |
| Sample Type: | Blood (plasma) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003170 |
| Treatment Summary: | the DCBI model included blast traumatic brain injury (bTBI), tissue injury through bilateral open femur fractures, followed by severe fixed pressure targeted hemorrhagic shock (HS). Zone-I aortic occlusion (AO) was performed using the ER-REBOA Catheter (Prytime Medical Devices, Inc., Boerne, TX). The catheter was inserted through a 7 Fr sheath placed in the common femoral artery, was advanced to 50 cm, and inflated using 6 mL of normal saline for Zone-I AO and placed at 26 cm with 5 ml of saline for Zone-III, and both were maintained for the final 30 minutes of the HS phase. |
Sample Preparation:
| Sampleprep ID: | SP003167 |
| Sampleprep Summary: | Plasma samples were thawed on ice then 20 μl was aliquoted into individual 1.5mL microcentrifuge tubes. The plasma was diluted to 480 μl with methanol:acetonitrile:water (5:3:2, v/v/v). The metabolite containing supernatant was transferred to individual auto sampler vials on ice. |
| Sampleprep Protocol Comments: | DOI https://doi.org/10.1007/978-1-4939-9236-2_2 |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN004996 | AN004997 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Phenomenex Kinetex C18 (150 x 2.1mm,1.7um) | Phenomenex Kinetex C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | area under curve | area under curve |
Chromatography:
| Chromatography ID: | CH003775 |
| Chromatography Summary: | The LC-MS solvents A and B, as well as the Flow gradient details are proprietary and will be made available upon request (angelo.dalesandro@cuanschutz.edu) |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Phenomenex Kinetex C18 (150 x 2.1mm,1.7um) |
| Column Temperature: | 45 |
| Flow Gradient: | - |
| Flow Rate: | 0.3mL/min |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004736 |
| Analysis ID: | AN004996 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Scanning in Full MS mode (2 μscans) from 90 to 900 m/z at 70,000 resolution, with 4 kV spray voltage, 45 sheath gas, 15 auxiliary gas(1). Following data acquisition, .raw files were converted to .mzXML using RawConverter then metabolites assigned and peaks integrated using ElMaven (Elucidata) in conjunction with an in-house standard library. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS004737 |
| Analysis ID: | AN004997 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Scanning in Full MS mode (2 μscans) from 90 to 900 m/z at 70,000 resolution, with 4 kV spray voltage, 45 sheath gas, 15 auxiliary gas(1). Following data acquisition, .raw files were converted to .mzXML using RawConverter then metabolites assigned and peaks integrated using ElMaven (Elucidata) in conjunction with an in-house standard library. |
| Ion Mode: | POSITIVE |
