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MB Sample ID: SA331409
Local Sample ID: | T47D veh_1 |
Subject ID: | SU003166 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003166 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
T47D veh_1 | SA331409 | FL039189 | Yes | Hormone treatment |
Collection:
Collection ID: | CO003159 |
Collection Summary: | Cells were washed with 150 mM ammonium acetate and flash frozen, before storage at -80deg C. |
Sample Type: | Breast cancer cells |
Treatment:
Treatment ID: | TR003175 |
Treatment Summary: | The ER+ breast cancer cell line was subject to 1 uM of the selective estrogen receptor degrader (SERD), fulvestrant, with vehicle control control. Cells were subjected to treatment for 7 days, before reseeding into 10 cm dishes at 3-5e6 cells/dish. |
Sample Preparation:
Sampleprep ID: | SP003172 |
Sampleprep Summary: | Standard lipidomics protocol. See protocol file. |
Sampleprep Protocol Filename: | A004_Shotgun_Lipidomics.PDF |
Combined analysis:
Analysis ID | AN005002 | AN005003 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Shimadzu LC-30 | Shimadzu LC-30 |
Column | Waters ACQUITY UPLC HSS T3 (50 x 2.1mm,1.8um) | Waters ACQUITY UPLC HSS T3 (50 x 2.1mm,1.8um) |
MS Type | ESI | ESI |
MS instrument type | Triple TOF | Triple TOF |
MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | counts | counts |
Chromatography:
Chromatography ID: | CH003780 |
Chromatography Summary: | Positive mode lipidomics LC-MSMS |
Instrument Name: | Shimadzu LC-30 |
Column Name: | Waters ACQUITY UPLC HSS T3 (50 x 2.1mm,1.8um) |
Column Temperature: | 55 |
Flow Gradient: | (Time : Pump B%) (0.01 : 40) (10.00 : 98) (17.00 : 98) (17.10 : 40) |
Flow Rate: | 0.4 ml/min |
Solvent A: | Acetonitrile / water (40:60, v/v) with 10 mM ammonium acetate |
Solvent B: | Acetonitrile / water / isopropanol (10 : 5 : 85 v/v) with 10 mM ammonium acetate |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS004742 |
Analysis ID: | AN005002 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
MS Comments: | Acquisition of MS/MS spectra was controlled by data dependent acquisition (DDA) function of the Analyst TF software. Lipids are identified using LIPIDBLAST package. Quantification of lipids is done by Multiquant software (AB-SCIEX). Normalization of the data for different lipid classes is performed based on a set of internal standards. |
Ion Mode: | POSITIVE |
Acquisition Parameters File: | LIP_15min_MSMS_Jenny_new_20min_mass100-1200_Valve_Positive.txt |
MS ID: | MS004743 |
Analysis ID: | AN005003 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
MS Comments: | Acquisition of MS/MS spectra was controlled by data dependent acquisition (DDA) function of the Analyst TF software. Lipids are identified using LIPIDBLAST package. Quantification of lipids is done by Multiquant software (AB-SCIEX). Normalization of the data for different lipid classes is performed based on a set of internal standards. |
Ion Mode: | NEGATIVE |
Acquisition Parameters File: | LIP_15min_MSMS_Jenny_New_20min_valve_Negative.txt |