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MB Sample ID: SA331411
| Local Sample ID: | Test pool |
| Subject ID: | SU003166 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003166 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Test pool | SA331411 | FL039190 | pooled | Hormone treatment |
Collection:
| Collection ID: | CO003159 |
| Collection Summary: | Cells were washed with 150 mM ammonium acetate and flash frozen, before storage at -80deg C. |
| Sample Type: | Breast cancer cells |
Treatment:
| Treatment ID: | TR003175 |
| Treatment Summary: | The ER+ breast cancer cell line was subject to 1 uM of the selective estrogen receptor degrader (SERD), fulvestrant, with vehicle control control. Cells were subjected to treatment for 7 days, before reseeding into 10 cm dishes at 3-5e6 cells/dish. |
Sample Preparation:
| Sampleprep ID: | SP003172 |
| Sampleprep Summary: | Standard lipidomics protocol. See protocol file. |
| Sampleprep Protocol Filename: | A004_Shotgun_Lipidomics.PDF |
Combined analysis:
| Analysis ID | AN005002 | AN005003 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu LC-30 | Shimadzu LC-30 |
| Column | Waters ACQUITY UPLC HSS T3 (50 x 2.1mm,1.8um) | Waters ACQUITY UPLC HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple TOF | Triple TOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | counts | counts |
Chromatography:
| Chromatography ID: | CH003780 |
| Chromatography Summary: | Positive mode lipidomics LC-MSMS |
| Instrument Name: | Shimadzu LC-30 |
| Column Name: | Waters ACQUITY UPLC HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Gradient: | (Time : Pump B%) (0.01 : 40) (10.00 : 98) (17.00 : 98) (17.10 : 40) |
| Flow Rate: | 0.4 ml/min |
| Solvent A: | Acetonitrile / water (40:60, v/v) with 10 mM ammonium acetate |
| Solvent B: | Acetonitrile / water / isopropanol (10 : 5 : 85 v/v) with 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004742 |
| Analysis ID: | AN005002 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| MS Comments: | Acquisition of MS/MS spectra was controlled by data dependent acquisition (DDA) function of the Analyst TF software. Lipids are identified using LIPIDBLAST package. Quantification of lipids is done by Multiquant software (AB-SCIEX). Normalization of the data for different lipid classes is performed based on a set of internal standards. |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | LIP_15min_MSMS_Jenny_new_20min_mass100-1200_Valve_Positive.txt |
| MS ID: | MS004743 |
| Analysis ID: | AN005003 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| MS Comments: | Acquisition of MS/MS spectra was controlled by data dependent acquisition (DDA) function of the Analyst TF software. Lipids are identified using LIPIDBLAST package. Quantification of lipids is done by Multiquant software (AB-SCIEX). Normalization of the data for different lipid classes is performed based on a set of internal standards. |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | LIP_15min_MSMS_Jenny_New_20min_valve_Negative.txt |
