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MB Sample ID: SA332020
| Local Sample ID: | 1699490 |
| Subject ID: | SU003181 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003181 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 1699490 | SA332020 | FL039277 | MZ | Genotype |
Collection:
| Collection ID: | CO003174 |
| Collection Summary: | This study utilized a previously reported twin study database comprising metabolomics and proteomics of erythrocytes in adult twins. The study was approved by the Human Subjects office of The University of Iowa Carver College of Medicine. Written informed consent was obtained from all participating subjects. Subjects qualified for participation by meeting criteria for autologous blood donation according to standard operating procedures of The University of Iowa DeGowin Blood Center. Five dizygotic (DZ) and 13 monozygotic (MZ) twin pairs participated in this study, but twin pairs were not required to donate samples concurrently. Standard health history and demographic information was obtained at the time of enrollment and informed consent. Whole venous blood (EDTA, Vacutainer® purple top blood collection tube, 8 ml) collected from participants prior to blood donation was centrifuged at 500 × g for 5 min, followed by removal of the plasma and buffy coat. RBCs were washed twice with cold isotonic saline solution. After washing, a 30 μl aliquot of the packed red blood cells (pRBCs) was removed for complete blood count (CBC) analysis (Sysmex XE-2100™ Automated Hematology System, Sysmex Corp). A 100 μl aliquot of pRBCs was lysed with 900 μl of nanopure water. Samples were thoroughly mixed and stored at −80 °C prior to proteomic and metabolomic analyses. |
| Sample Type: | Erythrocytes |
Treatment:
| Treatment ID: | TR003190 |
| Treatment Summary: | N/A |
Sample Preparation:
| Sampleprep ID: | SP003187 |
| Sampleprep Summary: | The sample preparation process was carried out using the automated MicroLab STAR® system from Hamilton Company. Recovery standards were added prior to the first step in the extraction process for QC purposes. Sample preparation was conducted using a proprietary series of organic and aqueous extractions to remove the protein fraction while allowing maximum recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by LC and one for analysis by GC. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen and dried under vacuum. Samples were then prepared for the appropriate instrument, either LC/MS or GC/MS. |
Combined analysis:
| Analysis ID | AN005022 |
|---|---|
| Analysis type | MS |
| Chromatography type | Combined GC/LC |
| Chromatography system | Thermo-Finnigan Trace DSQ (GC); Waters Acquity (LC) |
| Column | Combined GC/LC |
| MS Type | Other |
| MS instrument type | Other |
| MS instrument name | Thermo Velos LTQ Orbitrap; Thermo Trace DSQ |
| Ion Mode | UNSPECIFIED |
| Units | area under curve |
Chromatography:
| Chromatography ID: | CH003794 |
| Chromatography Summary: | Combined GC/LC: Results not separable into separate LC and GC data. |
| Methods Filename: | Instrumentation_details.txt |
| Instrument Name: | Thermo-Finnigan Trace DSQ (GC); Waters Acquity (LC) |
| Column Name: | Combined GC/LC |
| Column Temperature: | - |
| Flow Gradient: | - |
| Flow Rate: | - |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | Combined GC/LC |
MS:
| MS ID: | MS004761 |
| Analysis ID: | AN005022 |
| Instrument Name: | Thermo Velos LTQ Orbitrap; Thermo Trace DSQ |
| Instrument Type: | Other |
| MS Type: | Other |
| MS Comments: | Combined GC/LC analysis. Results not separable into GC and LC data. |
| Ion Mode: | UNSPECIFIED |
| Analysis Protocol File: | Instrumentation_details.txt |
