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MB Sample ID: SA333232
| Local Sample ID: | P32 |
| Subject ID: | SU003207 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003207 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| P32 | SA333232 | FL039530 | Kidney | Sample source |
| P32 | SA333232 | FL039530 | Renal cell carcinoma | Disease |
Collection:
| Collection ID: | CO003200 |
| Collection Summary: | Both bone marrow samples and RCC samples (primary kidney cancer samples) were collected at Seoul National University Hospital. Bone marrow samples were obtained from 17 patients diagnosed with acute myeloid leukemia (AML) from 2016 to 2019. RCC samples were obtained from 21 patients diagnosed with RCC from 2016 to 2021. |
| Sample Type: | Bone marrow (Blood); Kidney (Tissue) |
Treatment:
| Treatment ID: | TR003216 |
| Treatment Summary: | Not applicable. |
Sample Preparation:
| Sampleprep ID: | SP003213 |
| Sampleprep Summary: | The AML and RCC samples for metabolome analysis were prepared in accordance with instructions from Human Metabolome Technologies (HMT). |
Combined analysis:
| Analysis ID | AN005060 | AN005061 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent CE-TOFMS system | Agilent CE-TOFMS system |
| Column | Capillary: Fused silica capillary i.d. 50 μm × 80 cm | Capillary: Fused silica capillary i.d. 50 μm × 80 cm |
| MS Type | ESI | ESI |
| MS instrument type | Other | Other |
| MS instrument name | Agilent CE-TOFMS system | Agilent CE-TOFMS system |
| Ion Mode | UNSPECIFIED | UNSPECIFIED |
| Units | Relative peak area | Relative peak area |
Chromatography:
| Chromatography ID: | CH003821 |
| Chromatography Summary: | The compounds were measured in the Cation and Anion modes of CE-TOFMS based metabolome analysis. |
| Instrument Name: | Agilent CE-TOFMS system |
| Column Name: | Capillary: Fused silica capillary i.d. 50 μm × 80 cm |
| Column Temperature: | 20 ℃ |
| Flow Gradient: | 10 μL/min |
| Flow Rate: | 10 μL/min |
| Sample Injection: | Pressure injection 50 mbar, 10 sec |
| Solvent A: | 50% acetonitrile/50% water; internal standards (20 μM) |
| Solvent B: | - |
| Capillary Voltage: | 4,000 V (Cation Mode); 3,500 V (Anion Mode) |
| Running Buffer: | Cation Buffer Solution (p/n : H3301-1001); Anion Buffer Solution (p/n : I3302-1023) |
| Running Voltage: | 30 kV |
| Sheath Liquid: | HMT Sheath Liquid (p/n : H3301-1020) |
| Washing Buffer: | Cation Buffer Solution (p/n : H3301-1001); Anion Buffer Solution (p/n : I3302-1023) |
| Chromatography Type: | CE |
MS:
| MS ID: | MS004798 |
| Analysis ID: | AN005060 |
| Instrument Name: | Agilent CE-TOFMS system |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | For the acute myeloid leukemia (AML) samples, 354 peaks, covering 243 peaks from Cation mode and 111 peaks from Anion mode, were detected, and among them, 185 peaks were annotated on the basis of HMT’s standard library and ‘Known-Unknown’ peak library:Peaks detected in CE-TOFMS analysis were extracted using automatic integration software (MasterHands ver. 2.17.1.11 developed at Keio University) in order to obtain peak information including m/z, migration time (MT), and peak area. The peak area was then converted to relative peak area by the following equation. The peak detection limit was determined based on signal-noise ratio; S/N = 3. Relative Peak Area = Metabolite Peak Area / (Internal Standard Peak Area × Sample Amount) |
| Ion Mode: | UNSPECIFIED |
| MS ID: | MS004799 |
| Analysis ID: | AN005061 |
| Instrument Name: | Agilent CE-TOFMS system |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | For the renal cell carcinoma (RCC) samples, 363 peaks, covering 243 peaks from Cation mode and 120 peaks from Anion mode, were detected; the 363 peaks were annotated using the same libraries as the AML samples. Peaks detected in the CE-TOFMS analysis were extracted using automatic integration software (MasterHands ver. 2.19.0.2 developed at Keio University) in order to obtain peak information, which includes m/z, migration time (MT), and peak area. The peak area was then converted to relative peak area by the following equation. The peak detection limit was determined based on signal-noise ratio; S/N = 3. Relative Peak Area = Metabolite Peak Area / (Internal Standard Peak Area × Sample Amount) |
| Ion Mode: | UNSPECIFIED |
