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MB Sample ID: SA333301
| Local Sample ID: | 908 |
| Subject ID: | SU003209 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003209 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 908 | SA333301 | FL039585 | serum | Sample source |
| 908 | SA333301 | FL039585 | control | Factor |
Collection:
| Collection ID: | CO003202 |
| Collection Summary: | Blood was collected and centrifuged at 3,000 rpm for 10 min and stored at -80°C environment |
| Sample Type: | Blood (serum) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003218 |
| Treatment Summary: | mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks). |
Sample Preparation:
| Sampleprep ID: | SP003215 |
| Sampleprep Summary: | Samples were vortex for 1 min after adding 0.4 mL methanol; then they were centrifuged for 10 min at 12,000 rpm at 4^oC, and the supernatant was dried and resolved using 0.15 mL 2-chloro-l-phenylalanine (4 ppm) solution, and then filtered by 0.22 μm membrane. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN005064 | AN005065 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | LC | LC |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Orbitrap Exploris 120 | Thermo Orbitrap Exploris 120 |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | m/z values | m/z values |
Chromatography:
| Chromatography ID: | CH003824 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0~1 min, 2% B2; 1~9 min, 2%~50% B2; 9~12 min, 50%~98% B2; 12~13.5 min, 98% B2; 13.5~14 min, 98%~2% B2; 14~20 min, 2% B2 |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 0.1% formic acid in water (v/v) |
| Solvent B: | 0.1% formic acid in acetonitrile (v/v) |
| Chromatography Type: | LC |
MS:
| MS ID: | MS004802 |
| Analysis ID: | AN005064 |
| Instrument Name: | Thermo Orbitrap Exploris 120 |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | MS1 range was 100-1000 m/z, and the number of data-dependent scans per cycle was 4; MS/MS resolving power was 15000 FWHM, normalized collision energy was 30%, and dynamic exclusion time was automatic. The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment. |
| Ion Mode: | POSITIVE |
| MS ID: | MS004803 |
| Analysis ID: | AN005065 |
| Instrument Name: | Thermo Orbitrap Exploris 120 |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | MS1 range was 100-1000 m/z, and the number of data-dependent scans per cycle was 4; MS/MS resolving power was 15000 FWHM, normalized collision energy was 30%, and dynamic exclusion time was automatic. The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment. |
| Ion Mode: | NEGATIVE |
